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ViaFluor® Live Cell Microtubule Stains

Cell-permeant probes for imaging the microtubule cytoskeleton in live cells. Available with blue, green, or far-red fluorescence.

Product Attributes

Dye

ViaFluor® 405, ViaFluor® 488, ViaFluor® 647

Probe cellular localization

Cytoskeleton, Microtubules

Cell permeability

Membrane permeant

For live or fixed cells

For live/intact cells

Assay type/options

Long term staining (24-72h)

Colors

Blue, Green, Far-red

Concentration

1000X in DMSO

Storage Conditions

Store at 2 to 8 °C, Protect dye component from light

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Technology

Dye
Size
Catalog #
price
Qty
Trial size (10 uL)
50 uL
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Product Description

ViaFluor® Live Cell Microtubule Stains are cell-permeant probes for imaging the microtubule cytoskeleton in live cells. They are simple, rapid and sensitive stains that can be imaged without a wash step.

  • Cell-permeant fluorescent microtubule probes
  • Rapid, sensitive, no-wash stains for live cells
  • Available with blue, green, or far-red fluorescence
  • ViaFluor® 647 dye compatible with SIM or STED

ViaFluor® Live Cell Microtubule Stains bind to polymerized tubulin and may be used to visualize microtubules in live cells for up to 24 hours in immortalized cell types. However, the probes may show cytotoxicity and cell cycle arrest with longer incubation times. The stains are supplied as a 1000X stock solutions in DMSO, and include a vial of 100 mM VRP, an efflux pump inhibitor that may improve probe retention and staining in some cell types.

ViaFluor® Live Cell Microtubule Stains cannot be fixed after staining, and cannot be used with fixed cells or tissues. The probes are not predicted to bind bacterial or yeast microtubules. Learn more about our other Fluorescent Cellular Stains for nucleus, mitochondria, lysosomes, or see our selection of Microbiology Stains & Kits.

 

ViaFluor® Live Cell Microtubule Stains

DyeEx/EmCatalog no.Size (1000X
in DMSO)*
Features
ViaFluor® 405 408/452 nm70064-T10 uLBlue fluorescence for the DAPI channel
7006450 uL
ViaFluor® 488500/515 nm70062-T10 uLGreen fluorescence for the FITC channel
7006250 uL
ViaFluor® 647650/675 nm70063-T10 uLFar-red fluorescence for the Cy®5 channel
Compatible with SIM or STED
7006350 uL
* Supplied with 100 mM VRP in DMSO for optional use.
Cy5 is a registered trademark of Cytiva.

References

  1. BBA General Subjects, 1865(6), 129891(2021), DOI: 10.1016/j.bbagen.2021.129891
  2. Chem. Sci., 11(32), 8506(2020), DOI: 10.1039/D0SC02837A
  3. bioRxiv, 2021.04.22.440958(2021), DOI: 10.1101/2021.04.22.440958
 

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