ExoBrite™ STORM CD81 Antibody

Validated CD81 antibody for detection of EVs, formulated to minimize aggregation, and conjugated to high-performance CF® Dyes for STORM.

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500 uL

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Product Description

ExoBrite™ STORM CD81 Antibody is designed for detecting the EV marker CD81 using stochastic optical reconstruction microscopy (STORM). Conjugated to optimized CF® Dyes for superior STORM performance, the antibody and its buffer are also specifically formulated for reliable detection of isolated EVs.

Validated antibody for detecting EV marker CD81
Available with excellent STORM dyes CF®498, CF®568, CF®583R, and CF®647Plus
Proprietary buffer for enhanced signal-to-noise
Available in 4 colors for FITC, Cy®3, Texas Red®, and Cy®5 channels

The most common proteins used as EV markers are CD9, CD63, and CD81, members of the tetraspanin family. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of EVs, but their expression levels vary depending on the cell type of origin.

Superior performance for STORM imaging

Other commercially available antibodies for tetraspanin proteins CD9, CD63, and CD81 are generally not validated for isolated EVs or super-resolution imaging, and may require tedious optimization for your EV prep and staining protocol.

ExoBrite™ STORM Antibodies are powered by our industry-leading CF® Dyes for STORM. Available in CF®498, CF®568, CF®583R, and CF®647Plus, these dyes offer exceptional photochemical switching properties—delivering superior signal quality and image reconstruction for unmatched performance in STORM.

The labeled antibody was carefully selected and validated for robust detection of isolated EVs. In addition, the antibodies are provided in a proprietary buffer formulation for reduced antibody aggregation and brighter EV staining for optimal accuracy and signal-to-noise. ExoBrite™ STORM Antibodies are compatible with co-staining with other fluorescently labeled antibodies or for bead-bound EV staining protocols.

Cholera toxin subunit B (CTB) conjugated to high-performance CF® Dyes for STORM are also available, as well as tetraspanin antibodies optimized for detection of EVs by flow cytometry and western blotting.

Learn more about our CF® Dyes for super-resolution imaging, as well as our other ExoBrite™ stains, antibodies, and other reagents developed specifically for EV detection and analysis.

ExoBrite™ STORM Antibody Conjugates

Antibody	Ex/Em	Size	Conc.	Detection Channel	Catalog No.
ExoBrite™ STORM CF®498 CD9 Antibody	498/519 nm	500 uL	100 ug/mL	FITC	P003-498ST-500
ExoBrite™ STORM CF®568 CD9 Antibody	562/584 nm	500 uL	100 ug/mL	Cy®3	P003-568ST-500
ExoBrite™ STORM CF®583R CD9 Antibody	585/609 nm	500 uL	100 ug/mL	Texas Red®	P003-583RST-500
ExoBrite™ STORM CF®647Plus CD9 Antibody	652/668 nm	500 uL	100 ug/mL	Cy®5	P003-647PST-500
ExoBrite™ STORM CF®498 CD63 Antibody	498/519 nm	500 uL	100 ug/mL	FITC	P004-498ST-500
ExoBrite™ STORM CF®568 CD63 Antibody	562/584 nm	500 uL	100 ug/mL	Cy®3	P004-568ST-500
ExoBrite™ STORM CF®583R CD63 Antibody	585/609 nm	500 uL	100 ug/mL	Texas Red®	P004-583RST-500
ExoBrite™ STORM CF®647Plus CD63 Antibody	652/668 nm	500 uL	100 ug/mL	Cy®5	P004-647PST-500
ExoBrite™ STORM CF®498 CD81 Antibody	498/519 nm	500 uL	100 ug/mL	FITC	P005-498ST-500
ExoBrite™ STORM CF®568 CD81 Antibody	562/584 nm	500 uL	100 ug/mL	Cy®3	P005-568ST-500
ExoBrite™ STORM CF®583R CD81 Antibody	585/609 nm	500 uL	100 ug/mL	Texas Red®	P005-583RST-500
ExoBrite™ STORM CF®647Plus CD81 Antibody	652/668 nm	500 uL	100 ug/mL	Cy®5	P005-647PST-500
ExoBrite™ STORM CF®568 CD9 (Mouse) Antibody	562/584 nm	500 uL	100 ug/mL	Cy®3	P018-568ST-500
ExoBrite™ STORM CF®647Plus CD9 (Mouse) Antibody	652/668 nm	500 uL	100 ug/mL	Cy®5	P018-647PST-500
ExoBrite™ STORM CF®568 CD63 (Mouse) Antibody	562/584 nm	500 uL	100 ug/mL	Cy®3	P022-568ST-500
ExoBrite™ STORM CF®647Plus CD63 (Mouse) Antibody	652/668 nm	500 uL	100 ug/mL	Cy®5	P022-647PST-500
ExoBrite™ STORM CF®568 CD81 (Mouse/Rat) Antibody	562/584 nm	500 uL	100 ug/mL	Cy®3	P019-568ST-500
ExoBrite™ STORM CF®647Plus CD81 (Mouse/Rat) Antibody	652/668 nm	500 uL	100 ug/mL	Cy®5	P019-647PST-500

CY DYE is a registered trademark of Cytiva; Texas Red is registered trademark of Thermo Fisher Scientific.

Product Attributes

Antibody number

#P005

SwissProt

P60033

Antibody type

Primary

Clonality

Monoclonal

Host species

Mouse

Isotype

IgG1, kappa

Antibody reactivity (target)

CD81

Synonyms

CD81; Target of the antiproliferative antibody 1 (TAPA-1); Tetraspanin-28; Tspan-28

Species reactivity

Baboon, Cynomolgus monkey, Human, Mouse (low reactivity), Non-human primates

Human gene symbol

CD81

Entrez gene ID

975

Unigene

54457

Molecular weight

26 kDa

Antibody target cellular localization

Exosomes/EVs, Plasma membrane

Cell/tissue expression

Exosomes, Hepatocytes, Lymphocytes, Monocytes/macrophages

Verified antibody applications

Exosome staining (verified)

Positive control

MCF-7 cells, MCF-7 derived exosomes

Shipping condition

Room temperature

Antibody research areas

Exosomes/EVs

Antibody/conjugate formulation

Proprietary buffer containing 0.05% sodium azide

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light

Regulatory status

For research use only (RUO)

Product origin

Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.

Documents, Protocols, SDS and COA

Documents

Super Resolution Microscopy Flyer

ExoBrite Stains and Antibodies Brochure

Protocols

PI-ExoBrite-STORM-Antibody-Conjugates

SDS

SDS Primary Antibodies

COA

Request the Certificate of Analysis

FAQs

Exosome & EV Staining

Can other membrane stains besides ExoBrite™ True EV Membrane Stains be used to stain extracellular vesicles (EVs)?

While early studies of EVs attempted to use first-generation membrane dyes like DiI or PKH to stain EVs, more recently this class of dyes has been found to be largely unsuitable for EV staining due to their high degree of aggregation. Dye aggregation not only generates nonspecific particles that are indistinguishable from EVs in flow cytometry, but also results in poor EV labeling efficiency. Biotium developed the ExoBrite™ True EV Membrane Stains in response to our customers difficulties with using traditional membrane dyes to stain EVs. See our Literature Digest for more information.

Which of Biotium’s antibodies can be used to stain EVs?

We strongly recommend our ExoBrite™ Flow Antibody Conjugates for staining both purified or bead-bound EVs. The antibodies are validated and optimized to offer bright signal and low background. They are available against human or mouse CD9, CD63, and CD81 tetraspanin proteins.

Can extracellular vesicles (EVs) be stained with ExoBrite™ True EV Membrane Stains and antibodies concurrently?

Yes, EVs can be stained simultaneously with an ExoBrite™ True EV Membrane Stain and a fluorescent antibody.

With purified EVs, we have seen good results when EVs were stained in 500 mL of 1X ExoBrite™ plus 1 ug/mL fluorescent antibody. Please view our Product Information Sheet for detailed protocols.

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