ExoBrite™ True EV Membrane Stains

Elevate your EV research with ExoBrite™ True EV Membrane Stains. Offering unparalleled coverage and solubility, ExoBrite™ True EV Membrane Stains are genuine lipophilic membrane dyes that set a new standard in pan-EV labeling over classic dyes like PKH or DiO/DiI/DiD for EV staining.

Features

• Lipophilic membrane dyes designed for pan-EV labeling
• Superior alternative to common membrane dyes like PKH, DiO, DiI, DiD
• Near-complete coverage of EVs in a sample
• Broad compatibility with EVs isolated from different sources
• Compatible with antibody co-staining
• Available in 4 colors for Pacific Blue®, FITC, PE, and APC channels

Detect EVs, not dye aggregates

Membrane dyes like PKH, DiO, and DiI, while common tools for labeling EVs, can pose significant challenges when used for EV staining. For example, membrane dyes often have poor solubility and thus form aggregates that can be confused with EVs. ExoBrite™ True EV Membrane Stains were designed specifically to address the issues of membrane dye aggregation while remaining a true lipophilic membrane stain for pan-EV labeling.

ExoBrite™ True EV Membrane Stains are the best choice for general pan-EV staining. They demonstrate higher (near-complete) coverage of EVs over competitor membrane dyes in fluorescence nanoparticle tracking analysis (fNTA). The dyes also offer clear differentiation of EVs from non-specific particles during flow detection. ExoBrite™ True EV Membrane Stains labeled all EV types tested to date (see Validated EV Sources below).

Note: We do not recommend using ExoBrite™ True EV Membrane Stains to stain bead-bound EVs. For bead-bound EVs we recommend using ExoBrite™ CTB EV Stains or ExoBrite™ WGA EV Stains.

Significantly Greater Coverage of EVs Over Other Membrane Dyes Such as PKH, DiO, and DiI

SEC purified MCF-7-derived EVs were stained with three green membrane dyes: ExoBrite™ 515/540 True EV Membrane Stain, PKH67, and Neuro-DiO. The stained EVs were diluted and run on a ZetaView particle analyzer. Total EV number was measured in scatter mode, and fluorescently-stained EV number was measured with 488 nm excitation and 500 nm filter. The percentage of particles stained with the dye is shown in the plot. ExoBrite™ 515/540 True EV Membrane Stain showed nearly complete coverage of EVs, much higher than PKH67 or Neuro-DiO.

SEC purified MCF-7-derived EVs were stained with two orange membrane dyes: ExoBrite™ 555/575 True EV Membrane Stain, and DiI. The stained EVs were diluted and run on a ZetaView particle analyzer. Total EV number was measured in scatter mode, and fluorescently-stained EV number was measured with 520 nm excitation and 550 nm filter. The percentage of particles stained with the dye is shown in the plot. ExoBrite™ 555/575 True EV Membrane Stain showed nearly complete coverage of EVs, much higher than DiI.

Also view ExoBrite™ CTB, WGA, and Annexin EV Surface Stains

ExoBrite™ EV Surface Stains are fluorescent conjugates of probes for labeling EV membrane surface targets. The stains were designed to offer lower aggregation than competitor membrane dyes and are suitable for detecting bead-bound EVs, unlike ExoBrite™ True EV Membrane Stains and other hydrophobic membrane dyes. ExoBrite™ EV Surface Stains are available as cholera toxin subunit B (CTB), wheat germ agglutinin (WGA), or Annexin V conjugates. Biotium also offers a convenient ExoBrite™ EV Surface Stain Sampler Kit that includes each ExoBrite™ EV Surface Stains (CTB, WGA, and Annexin V) for assessing which stain offers the best coverage for the EV samples of interest.

Note: ExoBrite™ EV Stains have been found to label EVs derived from several tested cell lines (see Validated EV Sources below), but may not stain EVs from every source.

ExoBrite™ True EV Membrane Stains

Validated EV Sources for ExoBrite™ EV Surface Stains

Biotium also offers other validated ExoBrite™ reagents for flow cytometry, western blotting, or super-resolution imaging.

Learn about ExoBrite™ Antibody Conjugates for optimal detection of CD9, CD63, and CD81 EV markers by flow cytometry and western blotting. For super-resolution imaging by STORM, learn about our ExoBrite™ STORM CTB EV Staining Kits available in four CF® Dyes validated for STORM.