“Light-on” LysoView 555

70060
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Catalog #: 70060, 70060-T
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Product Description

Light-On LysoView™ 555 is a red fluorogenic lysosome dye with pH-dependent fluorescence. Light-On LysoView™ dye is unique among commercially available lysosomotropic dyes in that its fluorescence in cells is activated by exposure to UV excitation. In solution, the dye shows pH-dependent fluorescence that does not require UV activation. In cells, the dye initially shows low fluorescence, but brief exposure to UV excitation from a mercury arc lamp through a DAPI filter activates bright red fluorescence localizing to lysosomes.

We hypothesize that the dye assumes a non-fluorescent structure that can be switched to a fluorescent structure by UV excitation. Mercury arc lamp excitation of the dye using other filter sets (FITC, rhodamine) or dye excitation with a 405 nm laser does not activate fluorescence. Lysosomal fluorescence fades several minutes after UV exposure, but can be re-activated in the same cells multiple times by exposure to UV light . Therefore the dye provides a novel tool for UV-activated, reversible fluorescence imaging of lysosomes. See the product protocol under the downloads tab for more information.

Also see our red fluorescent LysoView™ 540 (catalog no. 70061), and far-red fluorescent LysoView™ 633 (catalog no. 70058) lysosome stains, which do not require UV activation.

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Additional Information

Size

10 uL, 50 uL

Flyer (PDF): LysoView and MitoView Dyes

MSDS (PDF): MSDS 70060

Protocol (PDF): PI-70060

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life