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Live Bacterial Gram Stain Kit

Wheat germ agglutinin binds specifically to N-acetylglucosamine in the peptidoglycan layer of gram-positive bacteria. The Live Bacterial Gram Stain Kit contains wheat germ agglutinin (WGA) conjugated to CF®594 to stain the surface of gram-positive cells with red fluorescence. The kit also includes DAPI to stain the DNA of all bacteria with blue fluorescence.

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Product Description

Wheat germ agglutinin binds specifically to N-acetylglucosamine in the peptidoglycan layer of gram-positive bacteria. The Live Bacterial Gram Stain Kit contains wheat germ agglutinin (WGA) conjugated to CF®594 to stain the surface of gram-positive cells with red fluorescence. CF®594 is spectrally similar to Texas Red®, but with brighter fluorescence. The kit also includes DAPI to stain the DNA of all bacteria with blue fluorescence. Gram-positive bacteria stained using this kit will show a fluorescent blue interior and fluorescent red cell surface, while gram-negative bacterial will be stained with blue fluorescence only.

 

Texas Red is a registered trademark of Thermo Fisher Scientific.

References

1.Science Journal of Chemistry (2018), doi:10.11648/j.sjc.20180605.11

 

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