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Live Bacterial Gram Stain Kit

A fluorescent staining kit for live cell gram staining in bacteria. Suitable for fluorescence microscopy and flow cytometry.

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200 assays
800 assays
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Supporting Documents

Product Description

The Live Bacterial Gram Stain Kit is a fluorescent staining kit for gram staining in bacteria. The assay is suitable for detection by fluorescence microscopy or flow cytometry.


  • A fluorescent assay for microscopy or flow cytometry
  • Simpler than traditional gram staining
  • Uses wheat germ agglutinin (WGA), which binds to peptidoglycans in gram-positive bacteria

Kit Components

  • CF®594 Wheat Germ Agglutinin (WGA)
  • DAPI, 125X Solution

Spectral Properties

  • CF®594 WGA Ex/Em 593/614 nm
  • DAPI Ex/Em 358/461 nm

WGA binds specifically to N-acetylglucosamine in the peptidoglycan layer of gram-positive bacteria. The Live Bacterial Gram Stain Kit contains WGA conjugated to CF®594 to stain the surface of gram-positive cells with red fluorescence. CF®594 is spectrally similar to Texas Red®, but with brighter fluorescence. The kit also includes DAPI to stain the DNA of all bacteria with blue fluorescence. Gram-positive bacteria stained using this kit will show a fluorescent blue interior and fluorescent red cell surface, while gram-negative bacteria will be stained with blue fluorescence only.

This kit is suitable for detection by fluorescence microscopy and flow cytometry.

We also offer the Bacterial Viability and Gram Stain Kit (32001) that also includes a dead-cell dye to simultaneously measure cell viability.

Biotium also offers BactoView™ Dead Stains and BactoView™ Viability Kits for highly-selective staining of dead bacteria, available in a selection of 7 colors from green to near-IR. BactoView™ Dead Stains also can be combined with fluorescent Gram stains like our CF® Dye WGA Conjugates.

BactoView™ Live Green and BactoView™ Live Red are designed for staining both live and dead bacteria. Note that BactoView™ Stains cannot be used to distinguish bacteria from eukaryotic cells, because they will stain other cell types as well. For bright and optimized labeling of bacterial endospores, see our BactoSpore™ Bacterial Stains.

See our full selection of Microbiology Stains & Kits.

Texas Red is a registered trademark of Thermo Fisher Scientific.


Science Journal of Chemistry (2018) 6;5 pp77-82 doi:10.11648/j.sjc.20180605.11


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