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BactoView™ Dead Stains

Highly-selective dead cell stains for bacteria cultures. The stains are suitable for both gram-positive and gram-negative strains.

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Product Description

BactoView™ Dead Stains are novel DNA binding dyes for live/dead discrimination in bacteria. These bright fluorogenic DNA binding dyes are cell membrane-impermeant, so they selectively stain dead bacteria with compromised cell membranes without the need to wash after staining.

  • Highly-selective dead cell stains for bacteria cultures
  • Compatible with both gram-positive and gram-negative strains
  • Quick 30-minute staining, no wash required
  • Available in 7 colors from green to near-IR

Highly-Selective Dead Cell Staining for Gram-Positive and Gram-Negative Strains

Traditional vital nucleic acid dyes like propidium iodide or ethidium homodimer are efficiently excluded from live gram-negative bacteria but are taken up by live gram-positive bacteria, resulting in high background in live cells and poor live/dead discrimination. BactoView™ Dead Stains have novel chemical structures that are efficiently excluded from both gram-positive and gram-negative strains, for highly selective live/dead discrimination. In addition to staining dead bacteria, BactoView™ Dead Stains also stain Bacillus subtilis endospores, but with dimmer fluorescence. BactoView™ Dead Stains have low fluorescence until they bind DNA, allowing bright, no-wash staining. The stains are available with a wide selection of emission wavelengths ranging from green to near-infrared, for microscopy or flow cytometry analysis.

BactoView™ Dead Stains & Viability Kits

Product NameEx/Em (nm)Detection ChannelSize (500X in DMSO)Catalog No.
BactoView™ Dead 500/515497/515FITC20 uL40107-T
100 uL40107
BactoView™ Dead 560/570559/570Rhodamine, PI, PE20 uL40108-T
100 uL40108
BactoView™ Dead 570/585572/583Rhodamine, PI, PE20 uL40109-T
100 uL40109
BactoView™ Dead 600/615603/613Texas Red® or PE-Texas Red®20 uL40110-T
100 uL40110
BactoView™ Dead 655/670653/671Cy®5, APC20 uL40111-T
100 uL40111
BactoView™ Dead 690/710683/707Cy®5.520 uL40112-T
100 uL40112
BactoView™ Dead 760/780759/780Cy®7, APC-Cy®720 uL40113-T
100 uL40113
BactoView™ Viability Kit (Green/Red)Green (498/522)/Red (572/583)FITC (Green)/Rhodamine, PI, PE (Red)1 kit32019
BactoView™ Viability Kit (Green/Far-Red)Green (498/522)/Far-Red (653/671)FITC (Green)/Cy®5, APC (Far-Red)1 kit32020
Texas Red is a registered trademark of Thermo Fisher Scientific; Cy Dye is a registered trademark of Cytiva.

Staining of Gram-Negative E. Coli

Untreated or heat-killed E. coli were stained with 1X BactoView™ Dead dye in PBS for 30 minutes at room temperature, then imaged by confocal microscopy without a wash step. BactoView™ Live Green panels (far right) are representative of the typical cell densities of untreated and heat-killed E. coli samples. Scale bars: 20 um.

Staining of Gram-Positive B. Subtilis

Untreated or heat-killed B. subtilis were stained with 1X BactoView™ Dead dye in PBS for 30 minutes at room temperature, then imaged by confocal microscopy without a wash step. BactoView™ Live Green panels (far right) are representative of the typical cell densities of untreated and heat-killed B. subtilis samples. Scale bars: 20 um.

BactoView™ Dead Also Stains B. Subtilis Endospores

B. subtilis were cultured in Difco sporulation medium (DSM) for 7 days to induce endospore formation. Spore cultures were washed in PBS and stained with 1X BactoView™ Dead stains in PBS for 30 minutes at room temperature, then imaged by confocal microscopy without a wash step. Scale bars: 20 um.

Biotium also offers BactoView™ Viability Kits, which include a choice of red or far-red BactoView™ Dead Stain for dead bacteria and BactoView™ Viability Green Counterstain to stain all bacteria. BactoView™ Dead Stains also can be combined with fluorescent Gram stains like our CF® Dye WGA Conjugates.

We also offer BactoView™ Live Green and BactoView™ Live Red for staining live bacteria. Note that BactoView™ Stains cannot be used to distinguish bacteria from eukaryotic cells, because they will stain other cell types as well. For bright and optimized labeling of bacterial endospores, see our BactoSpore™ Bacterial Stains.

For staining mammalian cells, see our NucSpot® Nuclear Stains for live/dead discrimination or nuclear counterstaining of fixed mammalian cells. For live nuclear staining of mammalian cells, see our NucSpot® Live Stains. Also, view our Cellular Stains Table for more information on how our dyes stain various organisms.

 

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