Monoclonal anti CD147 (8D6)

BNUB0424-0
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Product Description

This MAb recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.

Kirsch AH et. al. Tissue Antigens. 1997 Aug;50(2):147-52

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

CD147 (8D6)

Clonality

Monoclonal

Isotype

IgG1

Clone

8D6

Molecular weight

35 kDa (non-reduced); 40 kDa (reduced)

Synonyms
Entrez gene ID

682

SwissProt

P35613

Unigene

501293

Immunogen

Human T cell line Molt 13

Species reactivity

Human

Applications

Functional studies, Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cell surface

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 1:100-1:200, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT, Inhibits homotypic aggregation, adhesion to matrix, and migation thro ugh matrigel, order Ab without BSA/azide, Optimal dilution for a specific application should be determined, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

HSB2 cells. Renal Cell, Ovarian carcinoma or Melanoma

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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