Monoclonal anti EGFR (B1D8)

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Catalog #: BNUB0643
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Product Description

This antibody reacts with the extracellular domain of EGFR and blocks the EGF/TGF??induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, βcellulin and epiregulin.

Kudlow JE et. al. Journal of Biological Chemistry, 1986, 261(9):4134-8. | Bjorge JD et. al. Journal of Biological Chemistry, 1989, 264(7):4021-7. | Bjorge JD et. al. Journal of Biological Chemistry, 1987, 262(14):6615-22

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

EGFR (B1D8)

Clonality

Monoclonal

Isotype

IgG1

Clone

B1D8

Molecular weight

~170 kDa (wild type) and ~145 kDa (vIII variant)

Synonyms

Erbb1; ERBB1; Errp; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor Tyrosine Protein Kinase; ErbB1; Urogastrone; wa2; Wa5

Entrez gene ID

1956

SwissProt

P00533

Unigene

488293

Immunogen

Microsomes from A431 cells.

Species reactivity

Human

Applications

Affinity purification, Immunoprecipitation, Flow cytometry

Cellular localization

Cell surface

Application notes

Affinity Purification For coupling, order Ab without BSA, Does not react with mouse or rat; others not known, Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunoprecipitation 1-2 ug/500 ug protein lysate, Optimal dilution for a specific application should be determined by user

Positive control

A431 cells, Breast or Bladder cancer

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

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