Monoclonal anti Milk Fat Globulin (MFG 06)

BNUB0227-0
string(39) "$120.00"
Price:
 from
  $120.00
Clear selection
Catalog #: BNUB0227
Selection:
 Multiple sizes
Category: .
>>> Order 5 or more and receive 10% off.

Product Description

Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.

Larocca D et al. A Mr 46,000 human milk fat globule protein that is highly expressed in human breast tumors contains factor VIII-like domains. Cancer Res 1991, 51: 4994-4998 | Ceriani RL et al. Surface differentiation antigens of human mammary epithelial cells carried on the human milk fat globule. Proc Natl Acad Sci USA 1977, 74(2):582-6 | Corcoran D and Walker RA. Ultrastructural localization of milk fat globule membrane antigens in human breast carcinomas. J Pathol 1990,161(2):161-6 | Sterns EE et al. Prognostic significance of the immunohistochemical reaction to human milk fat globule antibodies in node-negative and node-positive breast cancer. Breast Cancer Res Treat 1992, 21(3):193-9 | Baildam AD et al. The expression of milk fat globule antigens within human mammary tumours: relationship to steroid hormone receptors and response to endocrine treatment. Eur J Cancer Clin Oncol 1989, 25(3):459-6

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Milk Fat Globulin (MFG-06)

Clonality

Monoclonal

Clone

MFG-06

Isotype

IgG1, kappa

Molecular weight

45 kDa

Synonyms

Breast epithelial antigen BA46; EDIL1; HMFG; Lactadherin; Lactadherin precursor; Medin; MFGE8; MFGM; Milk fat globule-EGF factor 8 (MFG-E8)

Entrez gene ID

4240

SwissProt

Q08431

Immunogen

Human milk fat globule membrane preparation

Unigene

3745

Species reactivity

Human

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cell Surface & Cytoplasmic

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 1-2 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 min at RT, Optimal dilution for a specific application should be determined, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

Normal breast or breast carcinoma

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life