Support & Resources

+1

MRP-14 Monoclonal Mouse Antibody (MRP14/840)

Recognizes a protein of14 kDa, identified as MRP-14 (also known as Calgranulin B or S100AA9).

Product Attributes

Antibody number

0840

Reactivity (target)

MRP-14

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

MRP14/840

Isotype

IgM, kappa

Molecular weight

14 kDa

Synonyms

S100-A9; MRP14; calgranulin-B; calprotectin L1H subunit; leukocyte L1 complex heavy chain; migration inhibitory factor-related protein 14; S100 calcium-binding protein A9 (calgranulin B)

Human gene symbol

S100A9

Entrez gene ID

6280 (S100A9 / Calgranulin B / MRP-14)

SwissProt

P06702 (S100A9 / Calgranulin B / MRP-14)

Unigene

112405 (S100A9 / Calgranulin B / MRP-14)

Immunogen

Recombinant human MRP14 protein

Cellular localization

Cytoplasmic

Species reactivity

Human

Applications

Immunofluorescence, Immunohistology (formalin), Flow cytometry

Application notes

Immunohistology formalin-fixed. Not suitable for frozen tissues. 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Immunofluorescence 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Optimal dilution for a specific application should be determined by user

Positive control

Tonsil, lymph node, or spleen

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Cancer, Transporters

Tumor expression

Multidrug resistant cancer

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Size
Catalog #
price
Qty
50 uL
100 uL
250 uL
500 uL
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

Recognizes a protein of14 kDa, identified as MRP-14 (also known as Calgranulin B or S100AA9). It comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. It is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore, it is also detectable in feces. Elevated levels have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions. This MAb reacts with neutrophils, monocytes, and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

References

Kong, J.P., et al. 2004. Loss of myeloid-related proteins 8 and myeloid- related proteins 14 expression in human esophageal squamous cell carcinoma correlates with poor differentiation. World J. Gastroenterol. 10: 1093-1097

 

You may also like…