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Calprotectin / Protein S100-A9 Monoclonal Mouse Antibody (CAGB/426)

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Product Attributes

Antibody number

#0035

Antibody reactivity (target)

Calprotectin, S100-A9

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

MAC387

Isotype

IgG1, kappa

Molecular weight

12-14 kDa (doublet)

Synonyms

Calprotectin; Protein S100-A9; Myeloid-related protein 14; MRP-14; Calgranulin B; CAGB; Calprotectin L1H Subunit

Entrez gene ID

6279 (S100A8/Calgranulin A/MRP-8) & 6280 (S100A9/Calgranulin B/MRP-14)

SwissProt

P05109 (S100A8/Calgranulin A/MRP-8) & P06702 (S100A9/Calgranulin B/MRP-14)

Unigene

416073 (S100A8/Calgranulin A/MRP-8) & 112405 (S100A9/Calgranulin B/MRP-14)

Immunogen

Affinity purified monocyte membrane preparation

Antibody target cellular localization

Cytoplasmic, Cytoskeleton

Cell/tissue expression

Granulocytes, Monocytes/macrophages

Species reactivity

Baboon, Cat, Cow, Dog, Goat, Guinea pig, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat

Verified antibody applications

IHC (FFPE) (verified)

Expected antibody applications

Flow (intracellular) (published for clone), IP (published for clone), WB (published for clone)

Antibody application notes

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 0.5-1 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Optimal dilution for a specific application should be determined by user

Positive control

Tonsil, lymph node, or spleen

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody research areas

Immunology, Inflammation

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Please be advised that primary antibodies with catalog numbers that begin with “BN” will only be offered conjugated to CF®405S, CF®488A, CF®568, CF®594, CF®640R, CF®647, and biotin as of 5/27/2022. However, previously available primary antibody conjugates that are in stock will remain available until inventory is exhausted. Please view the list of available conjugates on the product page. We also recommend our easy-to-use Mix-n-StainTM Antibody Labeling Kits for labeling antibodies with all CF® Dyes. We apologize for the inconvenience.
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Product Description

Calprotectin is a heteroduplex of Protein S100-A8/A9 (also called MRP-8/14, or Calgranulin A/B). This antibody recognizes the 14 kDa B component (Protein S100-A9) of the complex. Protein S100-A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. Altered expression of this protein is associated with the disease cystic fibrosis. This antibody reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

Catalog number key for antibody number 0035, Anti-Calgranulin-B (MAC387) 

Catalog Number Key for Primary Antibody Conjugates

Antibody # prefixConjugationEx/Em (nm)Laser lineDetection channelDye Features
BNC04CF®405S404/431405DAPI (microscopy), AF405CF®405S Features
BNC88CF®488A490/515488GFP, FITCCF®488A Features
BNC68CF®568562/583532, 561RFP, TRITCCF®568 Features
BNC94CF®594593/614561Texas Red®CF®594 Features
BNC40CF®640R642/662633-640Cy®5CF®640R Features
BNC47CF®647650/665633-640Cy®5CF®647 Features
BNCBBiotinN/AN/AN/A
BNUBPurifiedN/AN/AN/A
BNUMPurified,
BSA-free
N/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

References

Note: References for this clone sold by other suppliers may be listed for expected applications.

  1. J Histochem Cytochem (1987) 35(11): 1217-1226 (IP; WB; IHC, FFPE)
  2. J Histochem Cytochem (1990) 38(11): 1669-1675. (IHC, FFPE)
  3. J Leukocyte Biol (1994) 55: 259. (WB; IHC)
  4. World J Gastroenterol (2017)23(8): 1477-1488. (Flow, intracellular)
 

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