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Calgranulin B Monoclonal Mouse Antibody (CAGB/426)

Recognizes a 12-14 kDa doublet of Calgranulin A/B (also known as S100A8/A9 or MRP-8/14); expressed by granulocytes, monocytes and by tissue macrophages.

Product Attributes

Antibody number

0426

Reactivity (target)

S100A8/A9, MAC-387, MRP-8/-14, Calgranulin-B

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

CAGB/426

Isotype

IgM, kappa

Molecular weight

14 kDa

Entrez gene ID

6280 (S100A9 / Calgranulin B / MRP-14)

SwissProt

P06702 (S100A9 / Calgranulin B / MRP-14)

Human gene symbol

S100A9

Unigene

112405 (S100A9 / Calgranulin B / MRP-14)

Immunogen

Recombinant human CAGB protein

Cellular localization

Cytoplasmic

Cell/tissue expression

Epithelial cells, Monocytes/macrophages, Neutrophils

Species reactivity

Human

Applications

Immunofluorescence, Immunohistology (formalin), Flow cytometry

Application notes

Immunohistology formalin-fixed. Not suitable for frozen tissues. 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Immunofluorescence 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Optimal dilution for a specific application should be determined by user

Positive control

Tonsil, lymph node, or spleen

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Immunology, Inflammation

Conjugation
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Product Description

Recognizes a 12-14 kDa doublet of Calgranulin A/B (also known as S100A8/A9 or MRP-8/14); expressed by granulocytes, monocytes and by tissue macrophages.The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

References

Hermani, A., et al. 2005. Calcium-binding proteins S100A8 and S100A9 as novel diagnostic markers in human prostate cancer. Clin. Cancer Res. 11: 5146-5152

 

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