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Ep-CAM / CD326 Monoclonal Mouse Antibody (HEA125)

Product Attributes

Antibody number

#0825

Antibody reactivity (target)

CD326, Ep-CAM

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

HEA125

Isotype

IgG2b, kappa

Molecular weight

40-43 kDa

Synonyms

Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, Chromosome 17, ECS-1, EGP2, EGP314, EGP40, Epidermal Surface Antigen 1, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, ESA1, FLOT2, Flotillin-2, KSA, Membrane Component, REG-1, Reggie-1, Reggie-2, Surface Marker-1 (M17S1), TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1)

Human gene symbol

TACSTD1

Entrez gene ID

4072

SwissProt

P16422

Unigene

542050

Immunogen

Recombinant human EpCAM protein fragment from the extracellular domain (aa77-202) (exact sequence is proprietary)

Antibody target cellular localization

Exosomes/EVs, Membrane/cell surface

Species reactivity

Human

Verified antibody applications

Flow (surface) (verified), IF (verified), IHC (FFPE) (verified)

Expected antibody applications

RIA (published for clone), IHC (frozen) (published for clone), IP (published for clone)

Antibody application notes

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunohistology (formalin): 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min

Positive control

HT29 cells or Ovarian carcinoma

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Antibody research areas

Cancer, Cell adhesion, Exosomes/EVs

Cell/tissue expression

Epithelial cells

Tumor expression

Adenocarcinoma

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Please be advised Biotium will no longer be selling primary antibodies conjugated to R-PE, APC, PerCP, AP, or HRP as of 4/18/22. However, certain protein-labeled primary antibodies will remain available until inventory is exhausted. Please view the list of available conjugates on the product page. We also recommend our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with these conjugates. We apologize for the inconvenience.
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Product Description

This antibody recognizes a 40-43 kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. It has been reported that this epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

Catalog Number Key for Primary Antibody Conjugates

Antibody # prefixConjugationEx/Em (nm)Laser lineDetection channelDye Features
BNC04CF®405S404/431405DAPI (microscopy), AF405CF®405S Features
BNC05CF®405M408/452405DAPI (microscopy),
Pacific Blue™
CF®405M Features
BNC06CF®405L395/545405Pacific Orange™CF®405L Features
BNC88CF®488A490/515488GFP, FITCCF®488A Features
BNC14CF®514516/548488GFP, FITCCF®514 Features
BNC43CF®543541/560532RFP, TRITCCF®543 Features
BNC55CF®555555/565532, 561RFP, TRITCCF®555 Features
BNC68CF®568562/583532, 561RFP, TRITCCF®568 Features
BNC94CF®594593/614561Texas Red®CF®594 Features
BNC40CF®640R642/662633-640Cy®5CF®640R Features
BNC47CF®647650/665633-640Cy®5CF®647 Features
BNC60CF®660C667/685633-640Cy®5CF®660C Features
BNC61CF®660R663/682633-640Cy®5CF®660R Features
BNC80CF®680681/698633-640Cy®5.5, IR®700CF®680 Features
BNC81CF®680R680/701633-640Cy®5.5, IR®700CF®680R Features
BNC00CF®700695/720633-640AF700CF®700 Features
BNC70CF®770770/797785, 808IR®800CF®770 Features
BNCBBiotinN/AN/AN/A
BNUBPurifiedN/AN/AN/A
BNUMPurified,
BSA-free
N/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of GE Healthcare; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

References

Note: References for this clone sold by other suppliers may be listed for expected applications.

Br J Cancer (1987) 56: 714-721. (immunoprecipitation; RIA; IHC, frozen)

 

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