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Dilinoleyl DiI

Dilinoleyl DiI (1,1′-dilinoleyl-3,3,3′,3′-tetramethylindocarbocynanine perchlorate), also known as FAST DiI™, has identical absorption and emission spectra as DiI (#60010) .

Product Attributes

Cellular localization

Membrane/cell surface, Membrane/vesicular

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

Co-cultures, Extended staining (several days to weeks)

Fixation options

Permeabilize before staining, Fix after staining (formaldehyde), Fix before staining (formaldehyde)

Colors

Red

Excitation/Emission

549/565 nm

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Product Description

Dilinoleyl DiI (1,1′-dilinoleyl-3,3,3′,3′-tetramethylindocarbocynanine perchlorate), also known as FAST DiI™, has identical absorption and emission spectra as DiI™ (#60010) . Due to its unsaturated hydrocarbon chains, dilinoleyl DiI has a much faster lateral diffusion rate in cell membranes than DiI . This property makes dilinoleyl DiI particularly useful for tracing neurons in tissues. Also see Neuro-DiI.

  • λExEm (MeOH) = 549/565 nm
  • ε = 148,000
  • Dark red oily paste soluble in ethanol, DMF or DMSO
  • Store at -20°C and protect from light
  • C59H89ClN2O4
  • MW: 925.82

Chemical Structure:

 

Fast DiI is a trademark of Thermo Fisher Scientific.

References

1. Neuroreport 8, 3965 (1997).

2. Biophys J 70, 988 (1996).

3. J Cell Biol 134, 1427 (1996).

 

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