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NucSpot® Far-Red, 1000X in DMSO

An improved alternative to 7-AAD showing less bleed-through fluorescence in the PE-Texas Red® channel. Ideal for selective detection of dead cells by flow cytometry.

Product Attributes

Cellular localization

Nucleus

Applications

Flow cytometry

Assay type/options

DNA content/cell cycle (flow cytometry), No-wash staining

Cell permeability

Membrane impermeant

Apoptosis/viability marker

Dead cell stain

Colors

Far-red

Excitation/Emission

597/667 nm (with DNA)

Storage Conditions

Store at -10 to -35 °C

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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50 uL
0.5 mL
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Protocols
SDS

Product Description

NucSpot® Far-Red is an improved alternative to the popular flow cytometry dead cell dye 7-AAD. It has red-shifted fluorescence emission compared to 7-AAD, for less bleed-through fluorescence in the PE-Texas Red® channel.

  • Designed as an improved replacement for 7-AAD
  • Less bleed into the PE-Texas Red® channel compared to 7-AAD
  • Selective detection of dead cells by flow cytometry in the PE-Cy®5 or APC channel
  • Substitute for 7-AAD in any standard protocol
  • λExEm (with DNA) = 597/667 nm

Like 7-AAD, NucSpot® Far-Red can be excited with the 488 nm laser for detection in the PE-Cy®5 channel, or excited with the 633 nm laser for detection in the APC channel. NucSpot® Far-Red has similar brightness to 7-AAD in the PE-Cy®5 channel, and is much brighter in the APC channel.

NucSpot® Far-Red is excluded by live cells, but stains necrotic and late apoptotic cells with compromised membrane integrity. Like 7-AAD, NucSpot® Far-Red also can be used for DNA content analysis of cell cycle by flow cytometry in fixed and permeabilized cells without the need for RNase-treatment, unlike propidium iodide.

Cy dye is a registered trademark of GE Healthcare; Texas Red is a registered trademark of Thermo Fisher Scientific.
 

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