Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

NucSpot® Far-Red, 1000X in DMSO

NucSpot® Far-Red was designed to be an improved alternative to 7-AAD for selective detection of dead cells by flow cytometry.

Product Attributes

Cellular localization

Nucleus

Applications

Flow cytometry

Assay type/options

DNA content/cell cycle (flow cytometry), No-wash staining

Cell permeability

Membrane impermeant

Apoptosis/viability marker

Dead cell stain

Colors

Far-red

Size
Catalog #
price
Qty
50 uL
0.5 mL
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

Features

  • Designed as improved replacement for 7-AAD
  • Less bleed into the PE-Texas Red® channel compared to 7-AAD
  • Selective detection of dead cells by flow cytometry in the PE-Cy®5 or APC channel
  • Substitute for 7-AAD in any standard protocol
  • λExEm (with DNA) = 597/667 nm

NucSpot® Far-Red was designed to be an improved alternative to the popular flow cytometry dead cell dye 7-AAD. Like 7-AAD, it can be excited with the 488 nm laser for detection in the PE-Cy®5 channel, or excited with the 633 nm laser for detection in the APC channel. 7-AAD Plus has similar brightness to 7-AAD in the PE-Cy®5 channel, and is much brighter in the APC channel. Because its emission peak is red-shifted compared to 7-AAD, it has less bleed-through fluorescence in the PE-Texas Red® channel, making it useful for flow cytometry multiplexing.

NucSpot® Far-Red is excluded by live cells, but stains necrotic and late apoptotic cells with compromised membrane integrity. Like 7-AAD, NucSpot® Far-Red also can be used for DNA content analysis of cell cycle by flow cytometry in fixed and permeabilized cells without the need for RNase-treatment, unlike propidium iodide.

Cy dye is a registered trademark of GE Healthcare; Texas Red is a registered trademark of Thermo Fisher Scientific.
 

You may also like…