Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

RedDot™2 Far-Red Nuclear Stain, 200X in DMSO

RedDot™2 is a far-red cell membrane-impermeable nuclear dye similar to Draq7™, with excitation and emission at 665/695 nm. The dye is ideal for specifically staining the nuclei of dead cells with minimal cytoplasmic RNA staining.

Product Attributes

Cellular localization

Nucleus

For live or fixed cells

For fixed cells

Assay type/options

No-wash staining

Cell permeability

Membrane impermeant

Apoptosis/viability marker

Dead cell stain

Fixation options

Permeabilize before staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Colors

Far-red

Certificates of Analysis (COA)

Request COA
Size
Catalog #
price
Qty
25 uL (15-20 tests, trial size)
250 uL (150-200 tests)
1 mL (600-1000 tests)
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

RedDot™2 is a far-red cell membrane-impermeable nuclear dye similar to Draq7™, with excitation/emission at 665/695 nm for detection in the Cy®5 channel. The dye is ideal for specifically staining the nuclei of dead cells with minimal cytoplasmic RNA staining. RedDot™2 provides excellent nuclear counterstaining in fixed and permeabilized cells and tissue sections without the need for RNase treatment to remove cytoplasmic RNA. The dye is highly thermostable and photostable, providing convenient handling and ideal for demanding applications such as confocal microscopy. RedDot™2 also has been used for cell number normalization for near-infrared In Cell Western™ (see reference 5). RedDot™2 can be efficiently excited by wavelengths from 488 to 647 nm, and therefore can be used with the 488 nm flow cytometry laser line.

Please note: far-red dyes like RedDot™2 are not visible to the human eye, but must be imaged with a CCD camera or by confocal microscopy.

Please also see RedDot™1 (catalog no. 40060), a spectrally similar dye designed for specific nuclear staining of live cells.

In Cell Western is a trademark of LI-COR® Biosciences. Cy dye is a registered trademark of GE Healthcare. Draq5 is a trademark of BioStatus Ltd.

References

1. Environ Mol Mutagen (2013) doi: 10.1002/em.21817
2. PLoS Pathog (2013) doi:10.1371/journal.ppat.1003437
3. BMC Evolutionary Biology 13, 230 (2013)
4. Journal of International Society for Advancement of Cytometry (2013) doi: 10.1002/cyto.a.22410
5. PLoS ONE (2013) doi:10.1371/journal.pone.0077157
6. Journal of Biomedical Materials Research: Part A (2014), doi: 10.1002/jbm.a.35352
7. Cellular Microbiol. (2014), doi: 10.1111/cmi.12375
8. The Journal of Comparative Neurology Research in Systems Neuroscience (2015), doi: 10.1002/cne.23767
9. Biochimica & biophysica acta (2015), doi: 0.1016/j.bbamcr.2015.04.009
10. Journal of Neurotrauma (2015), doi: 10.1089/neu.2015.3899
11. Nature Communications (2015), doi: 10.1038/ncomms8222
12. Dev.Biol. (2015), http://dx.doi.org/10.1016/j.ydbio.2015.08.008i
13. Archives of Toxicology (2015), doi: 10.1007/s00204-015-1599-1
14. Molecular Neurodegeneration (2015), doi: 10.1186/s13024-015-0053-4
15. Modern Pathology (2016), doi:10.1038/modpathol.2016.52
16. Histopathology (2016), doi: 10.1111/his.13022
17. mBio (2017), doi: 10.1128/mBio.00007-17
18. Pathogens and Disease (2017), doi: https://doi.org/10.1093/femspd/ftx066
19. Cell Physiol Biochem (2017), doi: 10.1159/000480001
20. European Journal of Medicinal Chemistry (2017), https://doi.org/10.1016/j.ejmech.2017.11.004
21. Acta Neuropathologica Communications (2017), doi: 10.1186/s40478-017-0481-1
22. Environmental and Molecular Mutagenesis (2017), doi: 10.1002/em
23. Oncotarget. (2017), doi: 10.18632/oncotarget.16934.
24. Bio-protocol (2018), doi: 10.21769/Bioprotoc.2771
25. Environmental and Molecular Mutagenesis (2018), doi: 10.1002/em.22197
26. Stem Cell Research (2018), https://doi.org/10.1016/j.scr.2018.08.006
27. Molecular and Cellular Neuroscience (2018), doi:10.1016/j.mcn.2018.08.004

 

You may also like…

    NucSpot® Live Cell Nuclear Stains

    NucSpot® Live Cell Nuclear Stains are cell-membrane permeable DNA dyes that specifically stains nuclei in live or fixed cells. They have excellent specificity for DNA without the need for a wash step, and they have have low toxicity for live cell imaging. Supplied as 1000X stock solutions in DMSO.

    RedDot™1 Far-Red Nuclear Stain, 200X in H2O

    RedDot™1 is a far-red cell membrane-permeable nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells.

    DAPI, Dilactate

    DAPI dilactate is essentially the same as DAPI dihydrochloride (catalog no. 40011) but with a different counterion for improved water solubility.

    EverBrite™ Mounting Medium

    A revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes. Available with or without DAPI.

    Propidium Iodide (PI)

    Propidium iodide (PI) is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a cell population and also used as a counterstain in multicolor fluorescent imaging.

    Ethidium Homodimer III (EthD-III)

    Ethidium Homodimer III was developed by Biotium as an alternative to Ethidium Homodimer I. It has absorption and emission spectra similar to those of Ethidium Homodimer I, but stains DNA 45% brighter.