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RedDot™2 Far-Red Nuclear Stain, 200X in DMSO

A far-red cell membrane-impermeant nuclear dye similar to Draq7™. Ideal for fixed cell nuclear counterstaining with minimal cytoplasmic RNA staining.

Product Attributes

Cellular localization

Nucleus

For live or fixed cells

For fixed cells

Assay type/options

No-wash staining

Cell permeability

Membrane impermeant

Apoptosis/viability marker

Dead cell stain

Fixation options

Permeabilize before staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Colors

Far-red

Excitation/Emission

665 (broad)/695 nm (with DNA)

Concentration

200X in DMSO

Storage Conditions

Store at 2 to 8 °C, Protect from light

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

RedDot™2 is a far-red cell membrane-impermeant nuclear dye similar to Draq7™. Ideal for staining the nuclei of dead or fixed and permeabilized cells with minimal cytoplasmic RNA staining.

  • Ideal for specifically nuclear counterstaining of fixed cells or tissue sections
  • Minimal cytoplasmic RNA staining
  • Cell membrane-impermeant, for selective staining dead cells in culture
  • λExEm (DNA-bound) = 665/695 nm, for detection in the Cy®5 channel

RedDot™2 provides excellent nuclear counterstaining in fixed and permeabilized cells and tissue sections without the need for RNase treatment to remove cytoplasmic RNA. The dye is highly thermostable and photostable, providing convenient handling and ideal for demanding applications such as confocal microscopy. RedDot™2 can be efficiently excited by wavelengths from 488 to 647 nm, and therefore can be used with the 488 nm flow cytometry laser line. Please note: far-red dyes like RedDot™2 are not visible to the human eye, but must be imaged with a CCD camera or by confocal microscopy.

In unfixed cultures, RedDot™2 is dead cell-selective in all cell types tested, including mammalian cells, bacteria and yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms. Please also see RedDot™1 (catalog no. 40060), a spectrally similar dye designed for specific nuclear staining of live cells.



RedDot™1 and RedDot™2 Far-Red Nuclear Stains

ProductCatalog No.Cell PermeabilityLive/FixedColor (Ex/Em)Applications
RedDot™1 Far-Red Nuclear Stain, 200X in Water40060PermeantLive cells only(Far-red)
662/694 nm
• Live cell nuclear stain comparable to Draq5™
• For microscopy, cell cycle analysis by flow cytometry
• Can be used for In-Cell Western normalization
RedDot™2 Far-Red Nuclear Stain, 200X in DMSO40061ImpermeantFixed/permeabilized cells or tissues(Far-red)
665/695 nm
• Fixed cell nuclear stain comparable to Draq7™
• Nuclear-specific with minimal cytoplasmic staining
• Selectively stains dead cells in unfixed cultures

In Cell Western is a trademark of LI-COR® Biosciences. Cy dye is a registered trademark of GE Healthcare. Draq7 is a trademark of BioStatus Ltd.

References

Download a list of RedDot™2 References.

 

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