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RedDot™2 Far-Red Nuclear Stain

A far-red cell membrane-impermeant nuclear dye similar to Draq7™. Ideal for staining the nuclei of dead or fixed and permeabilized cells with minimal cytoplasmic RNA staining.

Product Attributes

Cellular localization


For live or fixed cells

For fixed cells

Assay type/options

No-wash staining

Cell permeability

Membrane impermeant

Apoptosis/viability marker

Dead cell stain

Fixation options

Permeabilize before staining, Fix before staining (methanol), Fix before staining (formaldehyde)




665 (broad)/695 nm (with DNA)

Catalog #
25 uL (200X in DMSO)
250 uL (200X in DMSO)
1 mL (200X in DMSO)
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Product Description

RedDot™2 is a far-red cell membrane-impermeant nuclear dye similar to Draq7™. Ideal for staining the nuclei of dead or fixed and permeabilized cells with minimal cytoplasmic RNA staining.

  • Cell membrane-impermeant
  • Ideal for specifically staining the nuclei of dead cells
  • Minimal cytoplasmic RNA staining
  • λExEm (DNA-bound) = 665/695 nm, for detection in the Cy®5 channel

RedDot™2 provides excellent nuclear counterstaining in fixed and permeabilized cells and tissue sections without the need for RNase treatment to remove cytoplasmic RNA. The dye is highly thermostable and photostable, providing convenient handling and ideal for demanding applications such as confocal microscopy. RedDot™2 can be efficiently excited by wavelengths from 488 to 647 nm, and therefore can be used with the 488 nm flow cytometry laser line.

RedDot™2 is dead cell specific in all cell types, including mammalian cells, bacteria and yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Please note: far-red dyes like RedDot™2 are not visible to the human eye, but must be imaged with a CCD camera or by confocal microscopy.

Please also see RedDot™1 (catalog no. 40060), a spectrally similar dye designed for specific nuclear staining of live cells.

RedDot™1 and RedDot™2 Far-Red Nuclear Stains

ProductCatalog No.Cell PermeabilityLive/FixedColor (Ex/Em)Applications
RedDot™1 Far-Red Nuclear Stain, 200X in Water40060PermeableLive cells only(Far-red)
662/694 nm
Comparable to Draq5™

For live cell staining with microscopy or cell cycle analysis by flow cytometry
RedDot™2 Far-Red Nuclear Stain, 200X in DMSO40061ImpermeableFixed/permeabilized cells or tissues(Far-red)
665/695 nm
Comparable to Draq7™

For fixed/permeabilized cells or tissues, or selective dead cell staining

In Cell Western is a trademark of LI-COR® Biosciences. Cy dye is a registered trademark of GE Healthcare. Draq7 is a trademark of BioStatus Ltd.


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