Coelenterazines are predominantly yellow solids, but may contain dark red or brown flecks. This does not affect product stability or performance. If your coelenterazine is uniformly brown, then it is oxidized and needs to be replaced.
Most of our products are stable at room temperature for many days, but we recommend storage at 4°C or -20°C to prolong shelf life. In the case of many of our aqueous dye solutions, the compounds are very stable at room temperature, but we recommend cold storage to prevent the growth of mold or other microbes over time. Therefore, to save on shipping costs, products with recommended storage at 4°C or -20°C may ship at ambient temperature or with an ice pack. These products may thaw without affecting product performance. When you receive the product, place it under the recommended storage conditions.
Biotium ships all antibodies (primary, secondary and conjugates) at room temperature. We guarantee their quality and performance under these conditions based upon our stability testing. Antibodies were subjected to accelerated stability testing by storing them at various temperatures (4°C, room temperature, or 37°C) for 1 week to mimic simulated shipping conditions and tested in immunostaining experiments. All antibodies showed the expected brightness and specificity, even after storage at sub-optimal temperatures.
In line with our goal to be more environmentally friendly by reducing the use of excess packaging, and lowering shipping costs for our customers, products that have passed our stability testing are shipped at room temperature.
Once you have received the antibody vial, please follow the long-term storage instructions on the product information (PI) sheet.
RedDot™2 is a far-red cell membrane-impermeant nuclear dye suitable for fixed cells or tissues, or selective dead cell staining. Unlike other far-red nuclear stains such as Draq7™, which can show significant cytoplasmic staining in permeabilized cells, RedDot™2 is nuclear-specific and offers highly selective nuclear counterstaining in fixed and permeabilized cells (Fig. 1).
Ideal for specifically nuclear counterstaining of fixed cells or tissue sections
Greater nuclear specificity than Draq7™, with minimal cytoplasmic RNA staining
Cell membrane-impermeant, for selective staining dead cells in culture
Highly thermostable and photostable, compatible with tissue clearing protocols
λEx/λEm (DNA-bound) = 665/695 nm, for detection in the Cy®5 channel
RedDot™2 provides excellent nuclear counterstaining in fixed and permeabilized cells and tissue sections without the need for RNase treatment to remove cytoplasmic RNA. The dye is highly thermostable and photostable, providing convenient handling and ideal for demanding applications such as confocal microscopy. RedDot™2 has also been validated for tissue clearing protocols such as CUBIC. RedDot™2 can be efficiently excited by wavelengths from 488 to 647 nm, and therefore can be used with the 488 nm flow cytometry laser line. Please note: far-red dyes like RedDot™2 are not visible to the human eye, but must be imaged with a CCD camera or by confocal microscopy.
In unfixed cultures, RedDot™2 is dead cell-selective in all cell types tested, including mammalian cells, bacteria and yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms. Please also see RedDot™1 (catalog no. 40060), a spectrally similar dye designed for specific nuclear staining of live cells.
A membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a mixed live-dead population and also used as a counterstain in multicolor fluorescent assays.