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RedDot™2 Far-Red Nuclear Stain, 200X in DMSO

RedDot™2 is a far-red cell membrane-impermeable nuclear dye similar to Draq7™, with excitation and emission at 665/695 nm. The dye is ideal for specifically staining the nuclei of dead cells with minimal cytoplasmic RNA staining.

Product Attributes

Cellular localization

Nucleus

For live or fixed cells

For fixed cells

Assay type/options

No-wash staining

Cell permeability

Membrane impermeant

Apoptosis/viability marker

Dead cell stain

Fixation options

Permeabilize before staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Colors

Far-red

Excitation/Emission

665 (broad)/695 nm (with DNA)

Size
Catalog #
price
Qty
25 uL (15-20 tests, trial size)
250 uL (150-200 tests)
1 mL (600-1000 tests)
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Product Description

RedDot™2 is a far-red cell membrane-impermeable nuclear dye similar to Draq7™. The dye is ideal for specifically staining the nuclei of dead cells with minimal cytoplasmic RNA staining. RedDot™2 provides excellent nuclear counterstaining in fixed and permeabilized cells and tissue sections without the need for RNase treatment to remove cytoplasmic RNA. The dye is highly thermostable and photostable, providing convenient handling and ideal for demanding applications such as confocal microscopy. RedDot™2 also has been used for cell number normalization for near-infrared In Cell Western™ (see reference 5). RedDot™2 can be efficiently excited by wavelengths from 488 to 647 nm, and therefore can be used with the 488 nm flow cytometry laser line.

RedDot™2 is dead cell specific in all cell types, including mammalian cells, bacteria and yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Product Features:

  • λExEm (DNA-bound) = 665/695 nm
  • Detect in the Cy®5 channel
  • Similar to Draq7™
  • Supplied at 200X in DMSO

Please note: far-red dyes like RedDot™2 are not visible to the human eye, but must be imaged with a CCD camera or by confocal microscopy.

Please also see RedDot™1 (catalog no. 40060), a spectrally similar dye designed for specific nuclear staining of live cells.

In Cell Western is a trademark of LI-COR® Biosciences. Cy dye is a registered trademark of GE Healthcare. Draq7 is a trademark of BioStatus Ltd.

References

1. Environ Mol Mutagen (2013) doi: 10.1002/em.21817
2. PLoS Pathog (2013) doi:10.1371/journal.ppat.1003437
3. BMC Evolutionary Biology 13, 230 (2013)
4. Journal of International Society for Advancement of Cytometry (2013) doi: 10.1002/cyto.a.22410
5. PLoS ONE (2013) doi:10.1371/journal.pone.0077157
6. Journal of Biomedical Materials Research: Part A (2014), doi: 10.1002/jbm.a.35352
7. Cellular Microbiol. (2014), doi: 10.1111/cmi.12375
8. The Journal of Comparative Neurology Research in Systems Neuroscience (2015), doi: 10.1002/cne.23767
9. Biochimica & biophysica acta (2015), doi: 0.1016/j.bbamcr.2015.04.009
10. Journal of Neurotrauma (2015), doi: 10.1089/neu.2015.3899
11. Nature Communications (2015), doi: 10.1038/ncomms8222
12. Dev.Biol. (2015), http://dx.doi.org/10.1016/j.ydbio.2015.08.008i
13. Archives of Toxicology (2015), doi: 10.1007/s00204-015-1599-1
14. Molecular Neurodegeneration (2015), doi: 10.1186/s13024-015-0053-4
15. Modern Pathology (2016), doi:10.1038/modpathol.2016.52
16. Histopathology (2016), doi: 10.1111/his.13022
17. mBio (2017), doi: 10.1128/mBio.00007-17
18. Pathogens and Disease (2017), doi: https://doi.org/10.1093/femspd/ftx066
19. Cell Physiol Biochem (2017), doi: 10.1159/000480001
20. European Journal of Medicinal Chemistry (2017), https://doi.org/10.1016/j.ejmech.2017.11.004
21. Acta Neuropathologica Communications (2017), doi: 10.1186/s40478-017-0481-1
22. Environmental and Molecular Mutagenesis (2017), doi: 10.1002/em
23. Oncotarget. (2017), doi: 10.18632/oncotarget.16934.
24. Bio-protocol (2018), doi: 10.21769/Bioprotoc.2771
25. Environmental and Molecular Mutagenesis (2018), doi: 10.1002/em.22197
26. Stem Cell Research (2018), https://doi.org/10.1016/j.scr.2018.08.006
27. Molecular and Cellular Neuroscience (2018), doi:10.1016/j.mcn.2018.08.004

 

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