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NucView® 405 Caspase-3 Substrate, 1 mM in PBS

NucView® 405 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

Product Attributes

Apoptosis/viability marker

Caspase

For live or fixed cells

For live/intact cells

Assay type/options

Long term staining (24-72h), Endpoint assay, No-wash staining, Real-time imaging, Homogenous assay

Detection method/readout

Fluorescence microscopy, Live cell imaging, Flow cytometry

Substrate specificity

Caspases

Colors

Blue

Fixation options

Fix after staining (formaldehyde), Permeabilize after staining

Size
Catalog #
price
Qty
Trial size (10 uL)
100 uL
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Product Description

NucView® 405 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, NucView® caspase-3 substrates can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. NucView® substrates consist of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with fluorescence. Thus, NucView caspase-3 substrates are bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. The staining is also formaldehyde-fixable.

NucView® 405  dye is excited by the 405 nm laser line for detection by confocal microscopy in the DAPI channel, or by flow cytometry in the Pacific Blue® channel. NucView® 405 is ideal for caspase-3 detection in multi-color applications for researchers who wish to reserve the green fluorescence channel for other detection reagents.

NucView® 405 Caspase-3 Substrate is offered as a 1 mM solution in DMSO (catalog no. 10405) or PBS (phosphate-buffered saline). The substrate in PBS is formulated for use in cells that are sensitive to DMSO toxicity. In non-DMSO sensitive cell types, adding DMSO during the substrate incubation may enhance NucView® 405 staining.

Note: NucView® 405 signal may be difficult to observe using epifluorescence microscopy. Confocal microscopy using 405 nm laser excitation is recommended for imaging. Biotium also offers green fluorescent NucView® 488 caspase-3 substrate and kits as well as orange fluorescent NucView® 530.

Pacific Blue is a registered trademark of Thermo Fisher Scientific.

References

n/a

 

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