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7-AAD (7-aminoactinomycin D) is a membrane-impermeant fluorescent DNA binding dye that is useful for live/dead discrimination and cell cycle profiling by flow cytometry.

Product Attributes

Probe cellular localization


Assay type/options

DNA content/cell cycle profiling (flow cytometry), Live/dead discrimination, No-wash staining

Detection method/readout

Flow cytometry

Cell permeability

Membrane impermeant

Apoptosis/viability marker

Dead cell stain




546/647 nm (with DNA)

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
Catalog #
1 mg
1 mL (1 mg/mL)
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Product Description

7-AAD is a fluorescent DNA binding dye that is membrane impermeant and therefore generally excluded from live cells and early apoptotic cells, but stains necrotic and late apoptotic cells with compromised membrane integrity.

  • Selective detection of dead cells by flow cytometry in the PE-Cy®5/PerCP channel
  • Perform cell cycle profiling by flow cytometry in fixed/permeabilized cells
  • λExEm (with DNA) = 546/647 nm (7-AAD can also be excited by the 488 or 532 laser lines)
  • Orange red solid soluble in DMF or DMSO
  • Store at -20°C and protect from light, especially in solution
  • C62H87N13O16
  • MW: 1270.45

7-AAD bound to DNA has a large Stokes shift with excitation/emission at 546/647 nm. The dye can be excited with either the 488 nm or 532 nm laser lines, and detected in the PE-Cy®5/PerCP flow cytometry channel. 7-AAD is widely used for live/dead cell discrimination. It also can be used in fixed and permeabilized cells for cell cycle profiling by DNA content analysis using flow cytometry. 7-AAD intercalates selectively at GC-rich regions of DNA, making it dye useful for chromosome banding studies. We offer 7-AAD as a solid powder (catalog no. 40037) or as a solution in 1 mg/mL DMSO:Water (1:1) (catalog no. 40084).

7-AAD products

ProductCatalog NumberUnit SizeFormat
7-AAD400371 mgOrange/red solid
7-AAD Solution, 1 mg/mL400841 mLRed solution in DMSO:Water (1:1)

We also offer the CF®488A Annexin V and 7-AAD Apoptosis kit, containing CF®488A Annexin V for staining apoptotic cells green, and 7-AAD for staining necrotic cells with far-red fluorescence. Also see NucSpot® Far-Red, an improved alternative to 7-AAD that has red-shifted fluorescence emission, for less bleed-through fluorescence in the PE-Texas Red® channel. For fixable dead cell stains, see our Live-or-Dye™ Fixable Viability Staining Kits.

Cy dye is a registered trademark of GE Healthcare; Texas Red is a registered trademark of Thermo Fisher Scientific.


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