PMA Real-Time PCR Bacterial Viability Kit – Mycobacterium tuberculosis (groEL2)
A kit designed to perform viability PCR on Mycobacterium tuberculosis. It contains the viability PCR dye PMA and primers to amplify the groEL2 gene.
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Product Description
This kit is designed to perform viability PCR on Mycobacterium tuberculosis. It contains the viability PCR dye PMA and primers to amplify the groEL2 gene.
Features
- Selectively detect viable Mycobacterium tuberculosis using qPCR
- Contains primers to amplify the groEL2 gene
- Works even in complex samples or mixed cultures
- Sufficient reagents to treat 80 bacterial cultures with PMA
- Sufficient reagents for 200 PCR reactions
Kit components
- PMA dye
- Forget-Me-Not™ EvaGreen® qPCR Master Mix
- ROX reference dye
- groEL2 primer mix, 5 uM
About this kit
Mycobacterium tuberculosis is a pathogenic bacteria that infects the lungs and causes the disease tuberculosis. PCR to detect Mycobacterium tuberculosis has been reported using the primers provided in the kit. In addition, these primers have been validated at Biotium for real-time qPCR using Forget-Me-Not™ EvaGreen® qPCR Master Mix. Note: groEL2 primers also amplify other mycobacteria species, but products may be distinguishable by melt curve analysis.
To learn more about the advantages of determining bacterial viability using viability PCR, visit the Viability PCR Technology Page.
Strain-Specific Bacterial Viability PCR Kits
| Bacteria Strain | Gene name | Kit with PMA (catalog #) | Kit with PMAxx™ (catalog #) |
|---|---|---|---|
| Salmonella enterica | invA | 31033 | 31033-X |
| Mycobacterium tuberculosis | groEL2 | 31034 | N/A |
| Staphylococcus aureus | nuc | 31035 | N/A |
| MRSA | mecA | 31036 | N/A |
| E. coli O157:H7 | Z3276 | 31037 | 31037-X |
| E. coli | uidA | 31050 | 31050-X |
| Listeria monocytogenes | hly | 31051 | 31051-X |
| Legionella pneumophila | mip | 31053 | N/A |
Don’t see your favorite strain? Let us know at techsupport@biotium.com.
References
Download PMA and PMAxx™ References and a list of PMA and PMAxx™ Validated Bacterial Strains.