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PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (mecA)

A kit designed to perform viability PCR on MRSA. It contains the viability PCR dye PMA and primers to amplify the mecA gene.

Product Attributes

Viability dye


Storage Conditions

Store at -10 to -35 °C

Assay type/options

Live/dead discrimination, Viability PCR

Detection method/readout


Catalog #
200 assays
Clear selection
(Please select quantity above )

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Product Description

This kit is designed to perform viability PCR on Methicillin-resistant Staphylococcus aureus. It contains the viability PCR dye PMA and primers to amplify the mecA gene.


  • Selectively detect viable Methicillin-resistant Staphylococcus aureus using qPCR
  • Contains primers to amplify the mecA gene
  • Works even in complex samples or mixed cultures
  • Sufficient reagents to treat 80 bacterial cultures with PMA
  • Sufficient reagents for 200 PCR reactions

Kit components

  • PMA dye
  • Forget-Me-Not™ EvaGreen® qPCR Master Mix
  • ROX reference dye
  • mecA primer mix, 5 uM

About this kit

Staphylococcus aureus is an anaerobic gram negative bacterium that can cause of a variety of infections in humans, and methicillin-resistant S. aureus is a rising cause of hospital-acquired infections. The mecA gene confers methicillin-resistance in S. aureus and other methicillin-resistant bacterial species. The mecA primers included in the kit have been used for detection of methicillin-resistant S. aureus, and have been validated for qPCR using Biotium’s Forget-Me-Not™ EvaGreen® qPCR Master Mix. Note that the mecA primers are not specific to S. aureus, because mecA is expressed by other methicillin-resistant bacteria. Biotium also offers the Staphylococcus aureus (nuc) PMA-PCR kit, which includes primers against the S. aureus-specific nuc gene, expressed by methicillin-susceptible and methicillin-resistant S. aureus strains.

To learn more about the advantages of determining bacterial viability using viability PCR, visit the Viability PCR Technology Page.

Strain-Specific Bacterial Viability PCR Kits

Bacteria StrainGene nameKit with PMA (catalog #)Kit with PMAxx™ (catalog #)
Salmonella entericainvA3103331033-X
Mycobacterium tuberculosisgroEL231034N/A
Staphylococcus aureusnuc31035N/A
E. coli O157:H7Z32763103731037-X
E. coliuidA3105031050-X
Listeria monocytogeneshly3105131051-X
Legionella pneumophilamip31053N/A

Don’t see your favorite strain? Let us know at

PMAxx technology is covered by granted and/or pending US and international patents.



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