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PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (mecA)

A kit designed to perform viability PCR on MRSA. It contains the viability PCR dye PMA and primers to amplify the mecA gene.

Product Attributes

Viability dye

PMA

Storage Conditions

Store at -10 to -35 °C

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200 assays
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Product Description

This kit is designed to perform viability PCR on Methicillin-resistant Staphylococcus aureus. It contains the viability PCR dye PMA and primers to amplify the mecA gene.

Features

  • Selectively detect viable Methicillin-resistant Staphylococcus aureus using qPCR
  • Contains primers to amplify the mecA gene
  • Works even in complex samples or mixed cultures
  • Sufficient reagents to treat 80 bacterial cultures with PMA
  • Sufficient reagents for 200 PCR reactions

Kit components

  • PMA dye
  • Forget-Me-Not™ EvaGreen® qPCR Master Mix
  • ROX reference dye
  • mecA primer mix, 5 uM

About this kit

Staphylococcus aureus is an anaerobic gram negative bacterium that can cause of a variety of infections in humans, and methicillin-resistant S. aureus is a rising cause of hospital-acquired infections. The mecA gene confers methicillin-resistance in S. aureus and other methicillin-resistant bacterial species. The mecA primers included in the kit have been used for detection of methicillin-resistant S. aureus, and have been validated for qPCR using Biotium’s Forget-Me-Not™ EvaGreen® qPCR Master Mix. Note that the mecA primers are not specific to S. aureus, because mecA is expressed by other methicillin-resistant bacteria. Biotium also offers the Staphylococcus aureus (nuc) PMA-PCR kit, which includes primers against the S. aureus-specific nuc gene, expressed by methicillin-susceptible and methicillin-resistant S. aureus strains.

To learn more about the advantages of determining bacterial viability using viability PCR, visit the Viability PCR Technology Page.

Strain-Specific Bacterial Viability PCR Kits

Bacteria StrainGene nameKit with PMA (catalog #)Kit with PMAxx™ (catalog #)
Salmonella entericainvA3103331033-X
Mycobacterium tuberculosisgroEL231034N/A
Staphylococcus aureusnuc31035N/A
MRSAmecA31036N/A
E. coli O157:H7Z32763103731037-X
E. coliuidA3105031050-X
Listeria monocytogeneshly3105131051-X
Legionella pneumophilamip31053N/A

Don’t see your favorite strain? Let us know at techsupport@biotium.com.

PMAxx technology is covered by granted and/or pending US and international patents.

References

Find a list of PMA and PMAxx™ references and a list of validated bacterial strains under Supporting Documents.

 

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