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PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (nuc)

A kit designed to perform viability PCR on Staphylococcus aureus. It contains the viability PCR dye PMA and primers to amplify the nuc gene.

Product Attributes

Viability dye

PMA

Storage Conditions

Store at -10 to -35 °C

Assay type/options

Live/dead discrimination, Viability PCR

Detection method/readout

PCR/qPCR

Size
Catalog #
price
Qty
200 assays
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Product Description

This kit is designed to perform viability PCR on Staphylococcus aureus. It contains the viability PCR dye PMA and primers to amplify the nuc gene.

Features

  • Selectively detect viable Staphylococcus aureus using qPCR
  • Contains primers to amplify the nuc gene
  • Works even in complex samples or mixed cultures
  • Sufficient reagents to treat 80 bacterial cultures with PMA
  • Sufficient reagents for 200 PCR reactions

Kit components

  • PMA dye
  • Forget-Me-Not™ EvaGreen® qPCR Master Mix
  • ROX reference dye
  • nuc primer mix, 5 uM

About this kit

Staphylococcus aureus is an anaerobic gram positive bacterium that can cause of a variety of infections in humans, and methicillin-resistant S. aureus is a rising cause of hospital-acquired infections. The primers included in this kit have been tested for amplification of the S. aureus-specific gene nuc gene from genomic DNA from both methicillin-susceptible and methicillin-resistant strains of S. aureus and have been validated at Biotium for real-time qPCR using Forget-Me-Not™ EvaGreen® qPCR Master Mix. Biotium also offers the Staphylococcus aureus (mecA) PMA-PCR kit, which includes primers against the methicillin-resistance gene mecA.

To learn more about the advantages of determining bacterial viability using viability PCR, visit the Viability PCR Technology Page.

Strain-Specific Bacterial Viability PCR Kits

Bacteria StrainGene nameKit with PMA (catalog #)Kit with PMAxx™ (catalog #)
Salmonella entericainvA3103331033-X
Mycobacterium tuberculosisgroEL231034N/A
Staphylococcus aureusnuc31035N/A
MRSAmecA31036N/A
E. coli O157:H7Z32763103731037-X
E. coliuidA3105031050-X
Listeria monocytogeneshly3105131051-X
Legionella pneumophilamip31053N/A

Don’t see your favorite strain? Let us know at techsupport@biotium.com.

PMAxx technology is covered by granted and/or pending US and international patents.

References

 

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