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PMA Real-Time PCR Bacterial Viability Kit – Legionella pneumophila (mip)

A kit designed to perform viability PCR on Legionella pneumophila. It contains the viability PCR dye PMA and primers to amplify the mip gene.

Product Attributes

Viability dye

PMA

Storage Conditions

Store at -10 to -35 °C

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200 assays
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Product Description

This kit is designed to perform viability PCR on Legionella pneumophila. It contains the viability PCR dye PMA and primers to amplify the mip gene.

Features

  • Selectively detect viable Legionella pneumophila using qPCR
  • Contains primers to amplify the mip gene
  • Works even in complex samples or mixed cultures
  • Sufficient reagents to treat 80 bacterial cultures with PMA
  • Sufficient reagents for 200 PCR reactions

Kit components

  • PMA dye
  • PMA Enhancer for Gram Negative Bacteria
  • Forget-Me-Not™ EvaGreen® qPCR Master Mix
  • ROX reference dye
  • mip primer mix, 5 uM

About this kit

Legionella pneumophila is a pathogenic species of gram-negative bacteria. It can infect the lungs and cause Legionnaire’s Disease. Legionella pneumophila is sometimes detected in water sources such as cooling towers and swimming pools. PCR to amplify the gene mip has been published and is highly specific for Legionella. The primers provided in the kit have been validated at Biotium for real-time qPCR using Forget-Me-Not™ EvaGreen® qPCR Master Mix.

To learn more about the advantages of determining bacterial viability using viability PCR, visit the Viability PCR Technology Page.

Strain-Specific Bacterial Viability PCR Kits

Bacteria StrainGene nameKit with PMA (catalog #)Kit with PMAxx™ (catalog #)
Salmonella entericainvA3103331033-X
Mycobacterium tuberculosisgroEL231034N/A
Staphylococcus aureusnuc31035N/A
MRSAmecA31036N/A
E. coli O157:H7Z32763103731037-X
E. coliuidA3105031050-X
Listeria monocytogeneshly3105131051-X
Legionella pneumophilamip31053N/A

Don’t see your favorite strain? Let us know at techsupport@biotium.com.

PMAxx technology is covered by granted and/or pending US and international patents.

References

Find a list of PMA and PMAxx™ references and a list of validated bacterial strains under Supporting Documents.

 

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