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PMA Real-Time PCR Bacterial Viability Kit – Mycobacterium tuberculosis (groEL2)

A kit designed to perform viability PCR on Mycobacterium tuberculosis. It contains the viability PCR dye PMA and primers to amplify the groEL2 gene.

Product Attributes

Viability dye

PMA

Storage Conditions

Store at -10 to -35 °C

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200 assays
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Product Description

This kit is designed to perform viability PCR on Mycobacterium tuberculosis. It contains the viability PCR dye PMA and primers to amplify the groEL2 gene.

Features

  • Selectively detect viable Mycobacterium tuberculosis using qPCR
  • Contains primers to amplify the groEL2 gene
  • Works even in complex samples or mixed cultures
  • Sufficient reagents to treat 80 bacterial cultures with PMA
  • Sufficient reagents for 200 PCR reactions

Kit components

  • PMA dye
  • Forget-Me-Not™ EvaGreen® qPCR Master Mix
  • ROX reference dye
  • groEL2 primer mix, 5 uM

About this kit

Mycobacterium tuberculosis is a pathogenic bacteria that infects the lungs and causes the disease tuberculosis. PCR to detect Mycobacterium tuberculosis has been reported using the primers provided in the kit. In addition, these primers have been validated at Biotium for real-time qPCR using Forget-Me-Not™ EvaGreen® qPCR Master Mix. Note: groEL2 primers also amplify other mycobacteria species, but products may be distinguishable by melt curve analysis.

To learn more about the advantages of determining bacterial viability using viability PCR, visit the Viability PCR Technology Page.

Strain-Specific Bacterial Viability PCR Kits

Bacteria StrainGene nameKit with PMA (catalog #)Kit with PMAxx™ (catalog #)
Salmonella entericainvA3103331033-X
Mycobacterium tuberculosisgroEL231034N/A
Staphylococcus aureusnuc31035N/A
MRSAmecA31036N/A
E. coli O157:H7Z32763103731037-X
E. coliuidA3105031050-X
Listeria monocytogeneshly3105131051-X
Legionella pneumophilamip31053N/A

Don’t see your favorite strain? Let us know at techsupport@biotium.com.

PMAxx technology is covered by granted and/or pending US and international patents.

References

Find a list of PMA and PMAxx™ references and a list of validated bacterial strains under Supporting Documents.

 

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