Thiazole orange (TO) is commonly used in reticulocyte analysis to stain residual RNA of blood cells1, to stain DNA in agarose gels2 and capillary electrophoresis3.
In live yeast, TO shows both nuclear concentration and cytoplasmic signal; after fixation the staining becomes completely cytoplasmic. In live mammalian cells, thiazole orange initially stains mitochondria and then redistributes to the nucleus and cytoplasm. Thiazole orange can also stain live bacteria (gram-positive and gram-negative). See our Cellular Stains Table for more information on how our dyes stain various organisms.
- λEx/λEm (with DNA) = 512/533 nm
- Supplied at 10 mM in DMSO
- Store at 4°C and protect from light
1. Cytometry 7, 508-517 (1986).
2. Nucleic Acids Res. 19, 327-333 (1990).
3. Chem. 66, 1941-1948 (1994).