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Thiazole Orange, 10 mM in DMSO

Thiazole orange (TO) is commonly used in reticulocyte analysis to stain residual RNA of blood cells, to stain DNA in agarose gels and capillary electrophoresis. Thiazole Orange stains the yeast nucleus, and is also able to stain bacteria and mammalian cells.

Product Attributes

Probe cellular localization

Nucleus & cytoplasm

For live or fixed cells

For live/intact cells

Assay type/options

No-wash staining

Cell permeability

Membrane permeant

Colors

Red

Excitation/Emission

512/533 nm (with DNA)

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1 mL
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Supporting Documents

Product Description

Thiazole orange (TO) is commonly used in reticulocyte analysis to stain residual RNA of blood cells1, to stain DNA in agarose gels2 and capillary electrophoresis3.

In live yeast, TO shows both nuclear concentration and cytoplasmic signal; after fixation the staining becomes completely cytoplasmic. In live mammalian cells, thiazole orange initially stains mitochondria and then redistributes to the nucleus and cytoplasm. Thiazole orange can also stain live bacteria (gram-positive and gram-negative). See our Cellular Stains Table for more information on how our dyes stain various organisms.

Product Features:

  • λExEm (with DNA) = 512/533 nm
  • Supplied at 10 mM in DMSO
  • Store at 4°C and protect from light

References

1.  Cytometry 7, 508-517 (1986).
2. Nucleic Acids Res. 19, 327-333 (1990).
3. Chem. 66, 1941-1948 (1994).

 

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