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Cell Surface & Membrane Dyes

Biotium offers two new classes of dyes for covalent labeling of the cell surface that are unique in that they can withstand fixation and permeabilization for subsequent IF staining. CellBrite™ Fix dyes are fluorogenic membrane dyes that covalently stain the plasma membrane in live cells. MemBrite™ Fix dyes covalently label cell surface proteins, and are available in with a wide selection of bright and photostable colors.

We also offer classic membrane and membrane potential dyes, including CellBrite™ and CellBrite™ NIR dyes, which are lipophilic carbocyanine dyes that stain the plasma membrane of cells and are non-toxic for long-term tracking.

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    Product

    CellBrite® Fix Membrane Stains

    From: $163 Sizes: Trial size (1 vial), Set of 5 vialsCatalog #:
    30089
    , 30089-T, 30088, 30088-T, 30090, - 30090-TView allHide

    Unique fluorogenic membrane dyes that covalently stain the plasma membrane in live cells, and can withstand both fixation and detergent permeabilization.

     
    Product

    MemBrite® Fix Cell Surface Staining Kits

    From: $188 Sizes: 100 labelings, 500 labelingsCatalog #:
    30092
    , 30092-T, 30093, 30093-T, 30094, 30094-T, 30095, 30095-T, 30096, 30096-T, 30097, 30097-T, 30098, 30098-T, 30099, 30099-T, 30101, 30101-T, 30102, 30102-T, 30103, 30103-T, 30104, - 30104-TView allHide

    Wide choice of dye colors for covalent staining the surface of live cells to conveniently visualize cell boundaries in immunofluorescence experiments.

     
    Product

    CellBrite® Cytoplasmic Membrane Dyes

    From: $159 Sizes: 1 mL, 50 assaysCatalog #:
    30024
    , 30021, 30022, - 30023View allHide

    Dye solutions of lipophilic carbocyanine dyes DiB, Neuro-DiO, DiI, and DiD for non-toxic labeling of cytoplasmic membranes.

     
    Product

    CellBrite® NIR Cytoplasmic Membrane Dyes

    From: $159 Sizes: 100 uLCatalog #:
    30070
    , 30077, 30078, - 30079View allHide

    Biotium’s CellBrite® NIR Cytoplasmic Membrane Dyes are ready-to-use dye stock solutions that can be added directly to normal culture media to uniformly label cell membranes with near-infrared lipophilic dyes.

     
    Product

    Concanavalin A (Con A) CF® Dye Conjugates

    From: $189 Sizes: 5 x 1 mgCatalog #:
    29015
    , 29016, 29017, 29018, 29019, 29020, 29058, 29074, 29075, - 29080View allHide

    A lectin labeled with our superior fluorescent CF® dyes is an effective and widely used cell surface stain for yeast, fungi, and mammalian cells.

     
    Product

    CellBrite® Steady Membrane Staining Kits

    From: $232 Sizes: 100 labelings, 500 labelingsCatalog #:
    30105-T
    , 30105, 30106-T, 30106, 30107-T, 30107, 30108-T, 30108, 30109-T, - 30109View allHide

    Cell surface staining kits with fluorescent membrane dye plus enhancer for imaging live cell surface for several hours to days.

     
    Product

    Datura Stramonium Lectin (DSL)

    From: $180 Sizes: 1 mLCatalog #:
    29096
    , 29097, 29098, 29099, 29100, - 29101View allHide

    DSL binds to (beta-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over single N-acetylglucosamine residues. Biotium offers DSL conjugated to biotin and a selection of 5 bright and photostable CF® Dyes.

     
    Product

    Lycopersicon Esculentum (Tomato) Lectin (LEL, TL)

    From: $190 Sizes: 1 mLCatalog #:
    29102
    , 29103, 29104, 29105, 29106, - 29107View allHide

    Tomato lectin (LEL, TL) CF® Dye conjugates that bind to [GlcNAc] 1,3-N-acetylglucosamine, glycophorin, and Tamm-Horsfall glycoprotein. LEL, TL is a commonly used marker for blood vessels and microglial cells in rodent and neuroscience research. Biotium offers tomato lectin conjugated to biotin and a selection of 5 bright and photostable CF® Dyes.

     
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    Phaseolus Vulgaris Leucoagglutinin (PHA-L)

    From: $180 Sizes: 1 mLCatalog #:
    29114
    , 29115, 29116, 29117, 29118, - 29119View allHide

    PHA-L triggers lymphocyte agglutination and is available conjugated to biotin and a selection of 5 bright and photostable CF® Dyes.

     
    Product

    Ulex Europaeus Agglutinin I (UEA I)

    From: $180 Sizes: 1 mLCatalog #:
    29108
    , 29109, 29110, 29111, 29112, - 29113View allHide

    UEA I binds to glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. The lectin is an established marker for human endothelial cells and has been used to differentiate between angiosarcomas and epithelial tumors. Biotium offers UEA I conjugated to biotin and a selection of 5 bright and photostable CF® Dyes.

     
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    Wheat Germ Agglutinin (WGA) Conjugates

    From: $84 Sizes: 1 mg, 5 x 1 mgCatalog #:
    29095-1
    , 29095, 29021, 29022, 29023, 29024, 29025, 29026, 29027, 29028, 29029, 29059, 29064, 29073, 29021-1, 29022-1, 29023-1, 29024-1, 29025-1, 29026-1, 29027-1, 29028-1, 29029-1, 29059-1, 29064-1, 29077-1, 29077, 29076-1, - 29076View allHide

    A wheat germ agglutinin (WGA) lectin labeled with Biotium’s CF® Dyes, biotin, or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine and stains yeast bud scars as well as the cell membrane of gram+ bacteria and mammalian cells.

     
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    1,6-Diphenyl-1,3,5-hexatriene

    From: $69 Sizes: 100 mgCatalog #: 60021

    1,6-Diphenyl-1,3,5-hexatriene (DPH) is almost non-fluorescent in water, but it exhibits a strong increase in fluorescence after intercalation into membranes and can be used as a probe for viscosity, polarity and lipid order.

     
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    Biotin-DHPE

    From: $267 Sizes: 10 mgCatalog #: 60022

    Biotin-DHPE (N-(Biotinoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt) can be used to couple avidin or streptavidin to cell membranes or liposomes.

     
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    Biotin-X-DHPE

    From: $267 Sizes: 5 mgCatalog #: 60023

    Biotin-X-DHPE (N-((6-(Biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine, triethylammonium salt) can be used to couple avidin or streptavidin to cell membranes or liposomes.

     
    Product

    DiA

    From: $212 Sizes: 50 mgCatalog #: 60013

    DiA 4-(4-Dihexadecylaminostyryl)-N-methylpyridinium iodide) is a green fluorescent membrane dye which diffuses much faster than DiO in cell membranes. DiA and DiI (60010) have been used together for two color membrane staining.

     
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    DiB

    From: $308 Sizes: 10 mgCatalog #: 60036

    DiB is a lipophilic cationically charged blue fluorescent dye.