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SynaptoGreen™ C4 (Equivalent to FM®1-43)

A widely used green fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the red fluorescent dye SynaptoRed™ (70021), synapses or neuromuscular junctions can be imaged independently in two colors.

Product Attributes

CAS number

149838-22-2

Cellular localization

Membrane/vesicular

For live or fixed cells

For live/intact cells

Assay type/options

Real-time imaging

Colors

Green

Excitation/Emission

480/598 nm (in membranes)

Size
Catalog #
price
Qty
5 mg
5 x 1 mg
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Product Description

A widely used green fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the red fluorescent dye SynaptoRed™ (70021), synapses or neuromuscular junctions can be imaged independently in two colors. Research results from the Tsien and Kay labs showed that brain slices can be stained with SynaptoGreen™ when SR101 (Sulforhodamine 101, 80101) or ADVASEP-7 (70029) is used to reduce the background staining. Biotium offers several Nerve Terminal Staining Kits for staining brain slices. Also see AM1-43 (70024), which is similar to SynaptoGreen™, but with a formaldehyde-fixable amine group.

SynaptoGreen™ also can be used to stain bacteria (gram-positive or gram-negative) and yeast, although in yeast it rapidly internalizes to vacuolar membranes. See our Cellular Stains Table for more information on how our dyes stain various organisms.

  • λExEm: 510/625 nm (in MeOH); 480/598 nm (in membranes)
  • Red solid soluble in water
  • C30H49Br2N3
  • MW: 612

FM is a registered trademark of Thermo Fisher Scientific.

References

1. J Neurosci 12, 363(1992).

2. Science 255, 200 (1992).

3. Neuron 24, 803 (1999).

4. Neuron 24, 809 (1999).

5. Curr Prot Neurosci (2008) doi: 10.1002/0471142301.ns0206s43

 

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