SynaptoGreen™ C4 (Equivalent to FM®1-43)
A widely used green fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the red fluorescent dye SynaptoRed™ (70021), synapses or neuromuscular junctions can be imaged independently in two colors.
A widely used green fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the red fluorescent dye SynaptoRed™ (70021), synapses or neuromuscular junctions can be imaged independently in two colors. Research results from the Tsien and Kay labs showed that brain slices can be stained with SynaptoGreen™ when SR101 (Sulforhodamine 101, 80101) or ADVASEP-7 (70029) is used to reduce the background staining. Biotium offers several Nerve Terminal Staining Kits for staining brain slices. Also see AM1-43 (70024), which is similar to SynaptoGreen™, but with a formaldehyde-fixable amine group.
SynaptoGreen™ also can be used to stain bacteria (gram-positive or gram-negative) and yeast, although in yeast it rapidly internalizes to vacuolar membranes. See our Cellular Stains Table for more information on how our dyes stain various organisms.
- Î»Ex/Î»Em: 510/625 nm (in MeOH); 480/598 nm (in membranes)
- Red solid soluble in water
- MW: 612
Nerve Terminal Dyes and Kits
|SynaptoGreen™ Dyes |
(Ex/Em ~480/660 nm in membranes)
|70020... 70053||View full list of SynaptoGreen™ Dyes|
|SynaptoRed Dyes™ |
(Ex/Em ~510/750 nm in membranes)
|70021... 70050||View full list of SynaptoRed™ Dyes|
|ADVASEP-7||70029||Sulfonated cyclodextrin that aids in removal of free dye during washes|
|SCAS||70037||Quenches extracellular fluorescences with fewer wash steps than ADVASEP-7|
|Sulforhodamine 101||80101||Red fluorescent dye that quenches extracellular fluorescence of SynaptoGreen™ dyes|
|Nerve Terminal Staining Kits|
|Nerve Terminal Staining Kit I||70030||Includes SynaptoGreen™ C4 and ADVASEP-7|
|Nerve Terminal Staining Kit II (A)||70031||Includes AM1-43 and ADVASEP-7|
|Nerve Terminal Staining Kit II (B)||70031-1||Includes AM1-43 and SCAS|
|Nerve Terminal Staining Kit III||70032||Includes SynaptoGreen™ C4 and Sulforhodamine 101|
|Nerve Terminal Staining Kit V||70034||Includes SynaptoRed™ C2 and ADVASEP-7|
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.
1. J Neurosci 12, 363(1992).
2. Science 255, 200 (1992).
3. Neuron 24, 803 (1999).
4. Neuron 24, 809 (1999).
5. Curr Prot Neurosci (2008) doi: 10.1002/0471142301.ns0206s43