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SynaptoGreen™ C4 (Equivalent to FM®1-43)

A widely used green fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the red fluorescent dye SynaptoRed™ (70021), synapses or neuromuscular junctions can be imaged independently in two colors.

Product Attributes

CAS number

149838-22-2

Probe cellular localization

Membrane/vesicular

For live or fixed cells

For live/intact cells

Assay type/options

Real-time imaging

Colors

Green

Excitation/Emission

480/598 nm (in membranes)

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

A widely used green fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the red fluorescent dye SynaptoRed™ (70021), synapses or neuromuscular junctions can be imaged independently in two colors. Research results from the Tsien and Kay labs showed that brain slices can be stained with SynaptoGreen™ when SR101 (Sulforhodamine 101, 80101) or ADVASEP-7 (70029) is used to reduce the background staining. Biotium offers several Nerve Terminal Staining Kits for staining brain slices. Also see AM1-43 (70024), which is similar to SynaptoGreen™, but with a formaldehyde-fixable amine group.

SynaptoGreen™ also can be used to stain bacteria (gram-positive or gram-negative) and yeast, although in yeast it rapidly internalizes to vacuolar membranes. See our Cellular Stains Table for more information on how our dyes stain various organisms.

  • λEx/λEm: 510/625 nm (in MeOH); 480/598 nm (in membranes)
  • Red solid soluble in water
  • C30H49Br2N3
  • MW: 612


Nerve Terminal Dyes and Kits

ProductCatalog No.Features
SynaptGreen™ Dyes
(Ex/Em ~480/660 nm in membranes)
70020... 70053View full list of SynaptoGreen™ Dyes
SynaptoRed Dyes™
(Ex/Em ~510/750 nm in membranes)
70021... 70050View full list of SynaptoRed™ Dyes
Background Reducers
ADVASEP-770029Sulfonated cyclodextrin that aids in removal of free dye during washes
SCAS70037Quenches extracellular fluorescences with fewer wash steps than ADVASEP-7
Sulforhodamine 10180101Red fluorescent dye that quenches extracellular fluorescence of SynaptoGreen™ dyes
Nerve Terminal Staining Kits
Nerve Terminal Staining Kit I70030Includes SynaptoGreen™ C4 and ADVASEP-7
Nerve Terminal Staining Kit II (A)70031Includes AM1-43 and ADVASEP-7
Nerve Terminal Staining Kit II (B)70031-1Includes AM1-43 and SCAS
Nerve Terminal Staining Kit III70032Includes SynaptoGreen™ C4 and Sulforhodamine 101
Nerve Terminal Staining Kit V70034Includes SynaptoRed™ C2 and ADVASEP-7
m is the number of carbons in the lipophilic tail and n is the number of double bonds linking the two aromatic rings in the dye.
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.
FM is a registered trademark of Thermo Fisher Scientific.

References

1. J Neurosci 12, 363(1992).

2. Science 255, 200 (1992).

3. Neuron 24, 803 (1999).

4. Neuron 24, 809 (1999).

5. Curr Prot Neurosci (2008) doi: 10.1002/0471142301.ns0206s43

 

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