Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

Dipicrylamine

Dipicrylamine (DPA) is a nonfluorescent anionic membrane dye. With its absorbance in the blue region of the visible spectrum, DPA can be used as a FRET quencher for a variety of fluorescent donor dyes.

Product Attributes

CAS number

131-73-7

For live or fixed cells

For live/intact cells

Potential dependence

Slow response (translational) membrane potential dye

Size
Catalog #
price
Qty
25 mg
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

Dipicrylamine (DPA) is a nonfluorescent anionic membrane dye. With its absorbance in the blue region of the visible spectrum, DPA can be used as a FRET quencher for a variety of fluorescent donor dyes. When applied to cells, DPA localizes at the interface of cytoplasmic membrane and aqueous phase in a manner sensitive to membrane potential change. A recent application of DPA is to measure cell membrane potential change by combining with the fluorescent donor dye DiOC16 (cat# 60038). The DiO/DPA system has been reported to produce a fluorescence signal change of >56% in HEK-293 cells and >25% in neuronal cultures and brain slices per 100 mV membrane potential change. Please also see the DiO/DPA Membrane Potential Detection Kit (30037)).

Yellow solid soluble in DMSO at >30mg/mL

  • λabs (MeOH) = 406 nm
  • Store at 4°C
  • MWt: 439.21
  • [131-73-7]

 

References

1. J Neurosci 29(29), 9197 (2009).

 

You may also like…

    RH421

    RH421 is a fast responding membrane potential dye, exhibiting >20% fluorescence change per 100 mV on neuroblastoma cells.

    RH237

    The styryl dye RH237 is a fast-responding potentiometric probe that is primarily used for functional imaging of neurons.

    RH795

    The styryl dye RH795 is a fast-responding potentiometric probe that is primarily used for functional imaging of neurons. The dye is spectrally similar to RH414 (61016), but exhibits different physiological effects during staining. For example, RH414 causes arterial constriction during cortex staining, while RH795 does not (see references 1,2).

    DiO/DPA Membrane Potential Detection Kit

    The DiO/DPA system detects cytoplasmic membrane potential changes using the principle of fluorescence resonance energy transfer (FRET), where DiO is a "stationary" FRET donor while DPA acts as a potentiometric FRET acceptor. The DiO/DPA system has been reported to produce a fluorescence signal change of >56% in HEK-293 cells and >25% in neuronal cultures and brain slices per 100 mV membrane potential change.

    Di-4-ANEPPS

    Di-4-ANEPPS is a fast-responding membrane potential dye. Changes in the membrane potential of the cell correlate with changes in the fluorescence excitation intensity of the dye.

    Nerve Terminal Staining Kits

    Nerve Terminal Staining Kits contain Biotium's nerve terminal dyes and accessories for washing away or quenching extracellular dye fluorescence.