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DiOC2(3)

DiOC2(3) (3,3′-Diethyloxacarbocyanine, iodide) has been used for measuring membrane potentials in bacteria. The green fluorescent dye forms red fluorescent aggregates with increasing membrane potential.

Product Attributes

CAS number

905-96-4

For live or fixed cells

For live/intact cells

Assay type/options

Real-time imaging

Potential dependence

Slow response (translational) membrane potential dye

Colors

Green

Excitation/Emission

482/497 nm

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Product Description

DiOC2(3) (3,3′-Diethyloxacarbocyanine, iodide) has been used for measuring membrane potentials in bacteria. The green fluorescent dye forms red fluorescent aggregates with increasing membrane potential. The spectral shifting phenomenon has been exploited to measure membrane potential ratiometrically.

  • Ex/Em (MeOH) = 482/497 nm
  • Orange solid soluble in DMSO or DMF
  • Store at 4°C and protect from light
  • C21H21IN2O2
  • MW: 460.31
  • [905-96-4]

 

References

1) Biophys J 56, 979 (1989).

 

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