Cell & Organelle Stains

A high affinity inhibitor of the nicotinic acetylcholine receptor in the neuromuscular junction. Blocks acetylcholine activity at the postsynaptic membrane. The biotin conjugate can be subsequently labeled with anti-biotin antibodies, avidin, or streptavidin for signal amplification.

Calcein is a highly negatively charged, water-soluble fluorescein derivative. Its fluorescence is nearly independent of pH in the range between 6.5 and 12.

Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation.

5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate is membrane-permeant and thus can be loaded into cells via incubation. The diacetate is readily hydrolyzed to 5-(and-6)-carboxy-2′,7′-dichlorofluorescein (51015) by intracellular esterases.

CF® Dye biocytin is a cell-impermeant, fixable polar tracer that is a conjugate of CF® Dye and biotin with an aldehyde-fixable primary amine. Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis.

A fluorescently labeled cAMP analog that can be used to probe cAMP receptors.

DASPEI (2-(4-(Dimethylamino)styryl)-N-ethylpyridinium iodide) is used to stain mitochondria in live cells.

Di-12-ANEPPQ is a fast-responding membrane potential dye. It is more hydrophobic than Di-8-ANEPPQ and is useful for potential-sensitive retrograde labeling of neurons.

Di-2-ANEPEQ, also called JPW1114, is a fast-responding membrane potential dye. It is highly water-soluble and is usually introduced into cells by microinjection.

Di-4-ANEPPS is a fast-responding membrane potential dye. Changes in the membrane potential of the cell correlate with changes in the fluorescence excitation intensity of the dye.

Di-8-ANEPPQ is a fast-responding membrane potential dye. It is more hydrophobic than Di-2-ANEPEQ and is useful for potential-sensitive retrograde labeling of neurons.

ANEPPS dyes are fast responding membrane potential dyes. Compared to Di-4-ANEPPS, Di-8-ANEPPS is better retained in the outer leaflet of cell plasma membranes and thus more suitable for long-term membrane potential studies. Di-8-ANEPPS is also more photostable and less phototoxic than Di-4-ANEPPS

DiA 4-(4-Dihexadecylaminostyryl)-N-methylpyridinium iodide) is a green fluorescent membrane dye which diffuses much faster than DiO in cell membranes. DiA and DiI (60010) have been used together for two color membrane staining.

DiB is a lipophilic cationically charged blue fluorescent dye.

DiBAC₄(3) is a translational membrane potential dye that redistributes within the cell membrane when membrane potential changes.

DiD (DiIC18(5); 1,1′-dioctadecyl-3,3,3′,3′- tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt) is a lipophilic carbocyanine dye similar to DiI, but with longer absorption and emission wavelengths.