Cell & Organelle Stains

Biotin ethylenediamine, hydrobromide (N-(5-(6-((biotinoyl)amino)hexanoyl)amino)pentylmaleimide) is identical to Neurobiotin™, a useful anterograde and transneuronal tracer.

Biotin ethylenediamine, hydrochloride is functionally equivalent to Neurobiotin™ (biotin ethylenediamine, hydrobromide, 90057).

Biotin-AM3-25 is a conjugate of AM3-25 and biotin. Membranes labeled with the probe can subsequently couple to avidin or streptavidin.

A biotin-conjugated cAMP analog that can be used to probe cAMP receptors.

A biotinylated cyclic nucleotide that is an important second messenger in many biological processes and has been used as a tracer in immunoassays.

Biotin-DHPE (N-(Biotinoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt) can be used to couple avidin or streptavidin to cell membranes or liposomes.

Biotin-X-DHPE (N-((6-(Biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine, triethylammonium salt) can be used to couple avidin or streptavidin to cell membranes or liposomes.

A high affinity inhibitor of the nicotinic acetylcholine receptor in the neuromuscular junction. Blocks acetylcholine activity at the postsynaptic membrane. The biotin conjugate can be subsequently labeled with anti-biotin antibodies, avidin, or streptavidin for signal amplification.

Calcein is a highly negatively charged, water-soluble fluorescein derivative. Its fluorescence is nearly independent of pH in the range between 6.5 and 12.

Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation.

5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate is membrane-permeant and thus can be loaded into cells via incubation. The diacetate is readily hydrolyzed to 5-(and-6)-carboxy-2′,7′-dichlorofluorescein (51015) by intracellular esterases.

CF® Dye biocytin is a cell-impermeant, fixable polar tracer that is a conjugate of CF® Dye and biotin with an aldehyde-fixable primary amine. Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis.

A fluorescently labeled cAMP analog that can be used to probe cAMP receptors.

DASPEI (2-(4-(Dimethylamino)styryl)-N-ethylpyridinium iodide) is used to stain mitochondria in live cells.

Di-12-ANEPPQ is a fast-responding membrane potential dye. It is more hydrophobic than Di-8-ANEPPQ and is useful for potential-sensitive retrograde labeling of neurons.

Di-2-ANEPEQ, also called JPW1114, is a fast-responding membrane potential dye. It is highly water-soluble and is usually introduced into cells by microinjection.