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SynaptoRed™ C2M (Equivalent to FM®5-95)

SynaptoRed™ C2M is a red fluorescent probe used to follow synaptic vesicles.

Product Attributes

Probe cellular localization

Membrane/vesicular

For live or fixed cells

For live/intact cells

Assay type/options

Real-time imaging

Colors

Far-red

Excitation/Emission

510/750 nm (see product description)

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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5 mg
5 x 1 mg
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Product Description

SynaptoRed™ C2M (also known as FM®5-95), is a red fluorescent probe used to follow synaptic vesicles. SynaptoRed™ C2M is slightly more water soluble than SynaptoRed™ C2 (equivalent to FM®4-64).

  • λExEm (membranes) = ~510/750 nm*
  • Dark purple solid soluble in water
  • MW: 565.43

* Excitation/emission settings of 515/640 nm have been used for detection of yeast vacuole staining with SynaptoRed™ C2 (FM®4-64), and should be applicable to SynaptoRed™ C2M.

Nerve Terminal Dyes and Kits

ProductCatalog No.Features
SynaptGreen™ Dyes
(Ex/Em ~480/660 nm in membranes)
70020... 70053View full list of SynaptoGreen™ Dyes
SynaptoRed Dyes™
(Ex/Em ~510/750 nm in membranes)
70021... 70050View full list of SynaptoRed™ Dyes
Background Reducers
ADVASEP-770029Sulfonated cyclodextrin that aids in removal of free dye during washes
SCAS70037Quenches extracellular fluorescences with fewer wash steps than ADVASEP-7
Sulforhodamine 10180101Red fluorescent dye that quenches extracellular fluorescence of SynaptoGreen™ dyes
Nerve Terminal Staining Kits
Nerve Terminal Staining Kit I70030Includes SynaptoGreen™ C4 and ADVASEP-7
Nerve Terminal Staining Kit II (A)70031Includes AM1-43 and ADVASEP-7
Nerve Terminal Staining Kit II (B)70031-1Includes AM1-43 and SCAS
Nerve Terminal Staining Kit III70032Includes SynaptoGreen™ C4 and Sulforhodamine 101
Nerve Terminal Staining Kit V70034Includes SynaptoRed™ C2 and ADVASEP-7
m is the number of carbons in the lipophilic tail and n is the number of double bonds linking the two aromatic rings in the dye.
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.
FM is a registered trademark of Thermo Fisher Scientific.

References

1. Science 297, 1525 (2002).
2. Cell 107, 605 (2001).

 

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