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SynaptoRed™ C2 (equivalent to FM®4-64)

A widely used red fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the green fluorescent dye SynaptoGreen™ (70020), synapses or neuromuscular junctions can be imaged independently in two colors. SynaptoRed™ has also been used to study endocytosis in Dictyostelia.

Product Attributes

CAS number

162112-35-8

Cellular localization

Membrane/vesicular

For live or fixed cells

For live/intact cells

Assay type/options

Real-time imaging

Colors

Far-red

Excitation/Emission

510/750 nm (see product description)

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5 mg
5 x 1 mg
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Product Description

A widely used red fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the green fluorescent dye SynaptoGreen™ (70020), synapses or neuromuscular junctions can be imaged independently in two colors. SynaptoRed™ has also been used to study endocytosis in Dictyostelia. Please also see AM4-64 (70025), which is similar to SynaptoRed™, but with a formaldehyde-fixable amine group.

SynaptoRed™ can also be used to stain bacteria (gram-positive or gram-negative) and yeast, although in yeast it rapidly internalizes to vacuolar membranes. See our Cellular Stains Table for more information on how our dyes stain various organisms.

  • λExEm (membranes) = ~510/750 nm*
  • Dark purple solid soluble in water
  • C30H45Br2N3
  • MW: 608
  • [162112-35-8]

* Excitation/emission settings of 515/640 nm have been used for detection of yeast vacuole staining with SynaptoRed C2 (FM®4-64) (Ref. 4). FM is a registered trademark of Thermo Fisher Scientific.

References

1. J Neurosci 12, 363 (1992).
2. Science 255, 200 (1992).
3. J Cell Biol 121, 1311 (1993).
4. J. Cell Biol 128, 779 (1995).

 

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