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SynaptoRed™ C2 (Equivalent to FM®4-64)

A widely used red fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the green fluorescent dye SynaptoGreen™ (70020), synapses or neuromuscular junctions can be imaged independently in two colors. SynaptoRed™ has also been used to study endocytosis in Dictyostelia.

Product Attributes

CAS number

162112-35-8

Probe cellular localization

Membrane/vesicular

For live or fixed cells

For live/intact cells

Assay type/options

Real-time imaging

Colors

Far-red

Excitation/Emission

510/750 nm (see product description)

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

A widely used red fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the green fluorescent dye SynaptoGreen™ (70020), synapses or neuromuscular junctions can be imaged independently in two colors. SynaptoRed™ has also been used to study endocytosis in Dictyostelia. Please also see AM4-64 (70025), which is similar to SynaptoRed™, but with a formaldehyde-fixable amine group.

SynaptoRed™ can also be used to stain bacteria (gram-positive or gram-negative) and yeast, although in yeast it rapidly internalizes to vacuolar membranes. See our Cellular Stains Table for more information on how our dyes stain various organisms.

  • λEx/λEm (membranes) = ~510/750 nm*
  • Dark purple solid soluble in water
  • C30H45Br2N3
  • MW: 608
  • [162112-35-8]

* Excitation/emission settings of 515/640 nm have been used for detection of yeast vacuole staining with SynaptoRed C2 (FM®4-64) (Ref. 4).

Nerve Terminal Dyes and Kits

ProductCatalog No.Features
SynaptGreen™ Dyes
(Ex/Em ~480/660 nm in membranes)
70020... 70053View full list of SynaptoGreen™ Dyes
SynaptoRed Dyes™
(Ex/Em ~510/750 nm in membranes)
70021... 70050View full list of SynaptoRed™ Dyes
Background Reducers
ADVASEP-770029Sulfonated cyclodextrin that aids in removal of free dye during washes
SCAS70037Quenches extracellular fluorescences with fewer wash steps than ADVASEP-7
Sulforhodamine 10180101Red fluorescent dye that quenches extracellular fluorescence of SynaptoGreen™ dyes
Nerve Terminal Staining Kits
Nerve Terminal Staining Kit I70030Includes SynaptoGreen™ C4 and ADVASEP-7
Nerve Terminal Staining Kit II (A)70031Includes AM1-43 and ADVASEP-7
Nerve Terminal Staining Kit II (B)70031-1Includes AM1-43 and SCAS
Nerve Terminal Staining Kit III70032Includes SynaptoGreen™ C4 and Sulforhodamine 101
Nerve Terminal Staining Kit V70034Includes SynaptoRed™ C2 and ADVASEP-7
m is the number of carbons in the lipophilic tail and n is the number of double bonds linking the two aromatic rings in the dye.
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.
FM is a registered trademark of Thermo Fisher Scientific.

References

1. J Neurosci 12, 363 (1992).
2. Science 255, 200 (1992).
3. J Cell Biol 121, 1311 (1993).
4. J. Cell Biol 128, 779 (1995).

 

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