Other Enzyme Substrates

DAB reacts with HRP in the presence of peroxide to yield an insoluble brown-colored product at locations where peroxidase-conjugated antibodies are bound to samples.

A simple, colorimetric assay for determining live cell numbers by absorbance on a microplate reader.

10-Acetyl-3,7-dihydroxyphenoxazine (Amplex® Red), is regarded as the best fluorogenic substrate for peroxidase and H₂O₂ because it is highly sensitive, specific and stable.

ABTS (2,2′ -Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), diammonium salt) is a very sensitive chromogenic peroxidase substrate widely used for ELISA applications. The substrate yields a water-soluble green colored product.

A pink derivative of BCIP that produces a pink colored (λ_max 540 nm) precipitate instead of the dark blue precipitate of BCIP. BCIP and derivatives are the most widely used chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins and are compatible with a variety of applications.

NBT and BCIP PINK are widely used together as a chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization. This kit contains 100 mg each of BCIP Pink (10006) and the precipitation inducer NBT (10008).

A red derivative of BCIP that produces a red (λ_max 565 nm) colored precipitate instead of the blue/violet precipitate of BCIP. BCIP and derivatives are the most widely used chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins and are compatible with a variety of applications.

A red derivative of BCIP that produces a red (λ_max 565 nm) colored precipitate instead of the dark blue precipitate of BCIP. BCIP and derivatives are the most widely used chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications.

A water soluble formulation of the most widely used chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization.

The most widely used chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization. This form is soluble in DMF.

BCIP/NBT are widely used together as a chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization. This kit contains 100 mg each of BCIP sodium salt (10001) and the oxidant NBT (Nitro blue tetrazolium, 10008).

For researchers wishing to investigate the suitability of our various chromogenic β-galactosidase substrates, we offer this sampler kit, which contains 5 mg each of the following five different substrates: X-Gal (10011), Red-β-D-Gal (10012), Rose-β-D-Gal (10013), Purple-β-D-Gal (10014) and Green-β-D-Gal (10015).

For researchers wishing to investigate the suitability of our various chromogenic β-glucuronidase substrates, we offer this sampler kit, which contains 5 mg each of the following three different substrates: X-GlcU, CHA, Red-β-D-GlcU, CHA, and Rose-β-D-GlcU, CHA.

The sampler kit contains 10 mg each of BCIP Na (10001), BCIP Pink (10006), BCIP Red (10004) and 250 mg of NBT (10008). NBT forms a co-precipitant and enhances the BCIP reaction.

5-Cyano-2,3-ditolyl tetrazolium chloride (CTC) has been used to evaluate the respiratory activity of many bacterial populations as well as populations undergoing drug efficacy evaluations.  Healthy cells respiring via the electron transport chain will absorb and reduce CTC into an insoluble, red fluorescent formazan product.