Reactive CF® Dyes, Other Reactive Dyes & Biotinylation Reagents

APTS is a very useful green fluorescent dye for labeling glycoproteins or sugar molecules in general. The labeling occurs via reductive amination which involves the formation of a Schiff base between the amine of APTS and the aldehyde or ketone of the sugar, followed by the reduction of the Schiff base linkage to a stable carbon-nitrogen bond.

5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5.

Mixed isomers of carboxytetramethylrhodamine (TAMRA) free acid. The dye can be coupled to primary or secondary amines via standard peptide chemistry.

5(6)-TAMRA SE (full name: 5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester (mixed isomers)) is the amine reactive form of TAMRA, one of the most commonly used red fluorescent dyes. The succinimidyl ester reacts readily with primary or secondary amines under mild conditions.

6-FAM, SE (full name: 6-Carboxyfluorescein, succinimidyl ester, single isomer) is the amine-reactive form of 6-carboxyfluorescein single isomer.

6-TRITC (Tetramethylrhodamine 6-isothiocyanate chloride) is spectrally similar to TAMRA (90022).

5-TRITC (Tetramethylrhodamine 5-isothiocyanate chloride) is spectrally similar to TAMRA (90022).

6-Carboxy-X-rhodamine is a single isomer, long wavelength rhodamine dye.

5-CR6G (full name: 5-Carboxyrhodamine 6G, hydrochloride, single isomer) is the free acid form of 5-carboxyrhodamine 6G single isomer.

ANTS (8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt) is a highly negatively charged dye with an amino group that can be coupled to an aldehyde or ketone group to form an unstable Schiff base. The Schiff base is usually chemically reduced by sodium borohydride (NaBH₄) or sodium cyanoborohydride (NaB(CN)H₃) to form a stable linkage (1).

6-CR6G (full name: 6-Carboxyrhodamine 6G, hydrochloride, single isomer) is the free acid form of 6-carboxyrhodamine 6G single isomer.

Free acid form of 6-TAMRA (6-carboxytetramethylrhodamine) single isomer.

Free acid form of 5-TAMRA (5-Carboxytetramethylrhodamine, single isomer).

6-ROX (6-Carboxy-X-rhodamine) is a single isomer, long wavelength rhodamine dye.

5-ROX (full name: 5-Carboxy-X-rhodamine, triethylammonium salt, single isomer) is a single isomer, long wavelength rhodamine dye.

5(6)-ROX, SE (full name: 5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester) is an amine-reactive long wavelength rhodamine dye.