Reactive CF® Dyes, Other Reactive Dyes & Biotinylation Reagents

CPM is a widely used blue fluorescent thiol-reactive dye that is essentially nonfluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step.

Biotium developed CPMTS as an alternative to the widely used thiol-reactive coumarin dye CPM (#91010).

Cyanine 555  tyramide is used for tyramide signal amplification (TSA), an horseradish peroxidase (HRP)-catalyzed high-density staining method for increasing immunofluorescence sensitivity.

Cyanine NS succinimidyl esters are non-sulfonated cyanine dyes that can be used to label proteins, oligonucleotides and nucleic acids.

DNP (dinitrophenol) tyramide conjugate can be used for tyramide signal amplification (TSA), an horseradish peroxidase (HRP)-catalyzed high-density staining method for increasing immunofluorescence sensitivity.

DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.

Eosin-5-isothiocyanate is a good photosensitizer and can be used to selectively label amines. It has a quantum yield of 0.

Eosin-5-maleimide is a good photosensitizer and can be used to selectively label thiols. It has a quantum yield of 0.

5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5.

Amine-reactive form of 5-carboxyfluorescein single isomer.

6-FAM, SE (full name: 6-Carboxyfluorescein, succinimidyl ester, single isomer) is the amine-reactive form of 6-carboxyfluorescein single isomer.

5-FAM-PEO₈ SE (full name: 5-Carboxyfluorescein-PEO₈ propionate succinimidyl ester) has an approximately 32 Angstrom water-soluble PEG spacer that separates fluorescein and the amine-reactive SE group.

FITC (fluorescein-5-isothiocyanate) is an amine-reactive fluorescein derivative for labeling proteins or other molecules containing primary amines.

Fluorescamine is nonfluorescent until it reacts with primary amines. The reaction is completed in miniseconds and can be used to detect amine-containing compounds including peptides and proteins.

Fluorescein cadaverine can be coupled to activated carboxy groups or sulfonyl chlorides.

Fluorescein hexylamine can be coupled to activated carboxy groups or sulfonyl chlorides. This product could be used as a direct replacement to fluorescein cadaverine (cat#: 92000).