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Cyanine 555 Tyramide

Cyanine 555  tyramide is used for tyramide signal amplification (TSA), an horseradish peroxidase (HRP)-catalyzed high-density staining method for increasing immunofluorescence sensitivity.

Product Attributes

Excitation/Emission

555/565 nm

Chemical reactivity (reacts with)

Tyrosine residues

Functional group

Tyramide

Assay type/options

Tissue staining

Storage Conditions

Store at -10 to -35 °C, Protect from light

Detection method/readout

Fluorescence microscopy

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

Cyanine 555  tyramide conjugate is used for TSA in multicolor immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH). The HRP-catalyzed method enables high-density labeling of a target protein or nucleic acid with Cyanine 555. Multiple tyramide conjugates are deposited onto tyrosines adjacent to the target for enhanced fluorescent signal. Cyanine 555 is structurally identical to Cy®3 from GE Healthcare.

  • Orange-red fluorescent TSA reagent identical to Cy®3
  • High-density labeling of a target protein or nucleic acid for enhanced immunofluorescence sensitivity
  • Especially suited for the detection of low abundance targets
  • Detection sensitivity of over 100-fold compared to conventional procedures
  • Enables multiplex multicolor detection

We also offer CF®555 tyramide , a superior alternate to Cy®3 with better photostability and brightness.

See also our other TSA reagents including CF® dye tyramidesReady-to-Use Tyramide Amplification Buffer, Tyramide Amplification Buffer Plus (an improved formulation for enhanced TSA sensitivity), and CF® Dye Tyramide Amplification Kits.

Learn more about Tyramide Signal Amplification.

Also see CF® Dye Tyramides, available with a wide selection of our bright and photostable CF® dyes.

Cy3 is a registered trademark of GE Healthcare.

 

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