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Fluorescent CF® Dyes CF®740

CF®740

Near-infrared dye with superior chemical stability for TSA and photostability for microscopy
CF® Dye Spectrum:
CF®350 Dye
CF®350 is a blue fluorescent dye
CF®405S Dye
Improved brightness & photostability for the 405 nm laser line
CF®405M Dye
Improved brightness & photostability for the 405 nm laser line
CF®430 & CF®440 Dyes
Photostable 405 nm-excitable dyes with green fluorescence
CF®405L Dye
A 405 nm-excitable dye with green fluorescence emission
CF®450 Dye
A spectrally unique, 405 nm-excitable green dye
CF®488A Dye
A superior green fluorescent dye
CF®503R Dye
A spectrally unique green dye ideal for spectral flow cytometry
CF®514 Dye
Alternative green fluorescent dye
CF®532 Dye
A bright green fluorescent dye for the 532 nm laser
CF®543 Dye
An orange fluorescent dye ideal for the 543 nm laser
CF®535ST Dye
A red fluorescent dye designed for STORM super-resolution imaging
CF®550R Dye
A spectrally unique orange/red dye ideal for spectral flow cytometry
CF®555 Dye
A bright and photostable orange-red dye
CF®568 Dye
Outshines Alexa Fluor®568
CF®570
Bright red fluorescent dye
CF®583 & CF®583R Dyes
Bright red fluorescent dyes
CF®594 Dye
Truly the brightest deep red dye
CF®597R Dye
Unique green-excited dye for STORM
RPE-Astral™616
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®620R Dye
A bright and photostable far-red dye
CF®633 Dye
The best dye for 633/635 laser lines
CF®640R Dye
A highly photostable far-red dye
CF®647 & CF®647Plus
Highly fluorescent far-red dyes
CF®660C & CF®660R
Superior alternatives to Alexa Fluor® 660
CF®680 & CF®680R
Two outstanding 680 nm-excitable dyes
CF®700 Dye
Brighter alternative to Alexa Fluor® 700
CF®710 Dye
Near-infrared CF® Dye with exceptional photostability
CF®725 Dye
Near-infrared CF® Dye with exceptional photostability
CF®740 Dye
Near-infrared CF® Dye with superior chemical stability for TSA and photostability for microscopy
RPE-Astral™775
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®750 Dye
Near-infrared CF® Dye
CF®770 Dye
Near-infrared CF® Dye
CF®790 Dye
Near-infrared CF® Dye
APC-Astral™813
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®800 Dye
Near-infrared CF® Dye
CF®820 Dye
Near-infrared CF® Dye
CF®850 Dye
Near-infrared CF® Dye
CF®870 Dye
Near-infrared CF® Dye

Near-IR CF®740 is a next-generation near-infrared dye with superior chemical stability for TSA and photostability for microscopy.  CF®740 is extensively validated and available in a range of formats, such as a reactive tyramide, with Biotium Choice primary antibodies, as a Mix-n-Stain™ Antibody Labeling Kit, and more.

See products available with CF®740 or view our full selection of near-IR CF® Dyes. Additional conjugates or reactive dyes may be added upon request; contact [email protected] to inquire.

Normalized absorbance and emission spectra of CF®740 goat anti-mouse conjugate in PBS.

CF®740 Technical Summary

  • Abs/Em Maxima: 742/767 nm
  • Extinction coefficient: 105,000
  • Molecular weight: ~ 900
  • Excitation laser line: 633 to 685 nm
  • Replaces: Alexa Fluor®750, Cy®7, DyLight® 750

 

CF®740 Features

  • Superior photostability for microscopy and chemical stability for TSA

CF®740 Offers Superior Stability in TSA Buffer for Automated Staining

 

For near-infrared detection using tyramide signal amplification (TSA), we recommend CF®740 tyramide. The dye was developed to offer superior stability in oxidizing amplification buffer among spectrally similar dyes, making it compatible with automated staining platforms (e.g., BOND RX), and its narrow absorption peak minimizes channel spillover.

 

CF®740 free acid or Alexa Fluor® 750 free acid was diluted to 2 uM in 1X Tyramide Amplification Buffer with 0.0015% hydrogen peroxide and incubated at room temp... See More

Helpful Tools & Resources

Visualize and export the fluorescent dye spectrum of any CF® Dye or other dyes from Biotium.
Learn the history of fluorophores in life science research as well as the development and advantages of CF® Dyes.
Check out our handy fluorophore selection tool to help choose the best CF® Dye for your next experiment.
Download our single page reference guide for all CF® Dye fluorophores. Includes recommended channels, applications, relative brightness, and photostability.
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CF®750 Dye

CF is a registered trademark of Biotium, Inc; Alexa Fluor is a registered trademark of Thermo Fisher Scientific.

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