MiniMab™ Single-Domain Antibodies

What are Single-Domain Antibodies?

Single-domain antibodies (SdAbs), also known as VHH domains, are small antigen-binding fragments derived from the variable domain of heavy chain antibodies (HCAbs) that are found in camelids such as alpacas, llamas, and camels.

Conventional antibodies, such as mouse, rabbit, or rat IgGs, are heterodimers composed of two heavy and two light chains. Their antigen-binding site forms through the interaction of variable domains from both chains. In contrast, camelid HCAbs (IgG2 and IgG3 subclasses) lack light chains and the CH1 domain, relying on a single variable domain for antigen recognition.

Unlock Deeper Insights with MiniMab™ SdAbs

MiniMab™ single-domain antibodies (SdAbs) from Biotium are a next-generation labeling tool that leverage the power of SdAbs for superior performance in immunofluorescence and other applications. Their smaller ~15 kDa size relative to conventional antibodies enables deeper tissue penetration, faster staining times, and improved access to tightly packed or masked epitopes—making them ideal for high-resolution and super-resolution imaging. MiniMabs™ also offer minimal epitope-dye displacement, preserving spatial accuracy for techniques like STORM and other super-resolution methods.

Best-in-Class CF® Dyes for IF & Super-Resolution Applications

MiniMab™ SdAbs are available conjugated to Biotium’s industry-leading CF® Dyes, known for their exceptional brightness, photostability, and wide spectral coverage—including near-infrared options like CF®740. These dye conjugates allow for longer imaging sessions with minimal signal loss, producing consistently sharp and vivid results. With MiniMab™ CF® Dye conjugates, researchers gain a powerful and precise toolset for advancing imaging applications, from routine fluorescence microscopy to cutting-edge nanoscale visualization.