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Can cells be fixed after staining with MitoView™ dyes? Can MitoView™ dyes be used to stain cells or tissue sections that are already fixed?

Most of our MitoView™ dyes are sensitive to mitochondrial membrane potential, and therefore can be used in live cells only.

MitoView™ Green is relatively insensitive to mitochondrial membrane potential, and can be used to stain formaldehyde-fixed cells (for best results, we recommend first fixing, then staining). MitoView™ Green does not stain well with samples that are fixed with methanol, permeabilized with detergent, or paraffin embedded. For best results staining mitochondria in fixed cells or tissue sections, we recommend using one our Mitochondrial Marker Antibodies, available with a wide selection of bright and photostable CF® dyes and other conjugations.

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