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Can the labeling reaction tolerate the presence of BSA or gelatin?

While labeling antibody without stabilizer proteins generally gives the best results, but antibodies with BSA or gelatin can be labeled with Mix-n-Stain™ CF® Dye Antibody Labeling Kits. The BSA or gelatin will also be labeled, but due to the high solubility of CF® dyes, the labeled BSA or gelatin is washed away during standard immunofluorescence staining. We recommend downloading the updated Product Protocol and completing the pre-labeling checklist to find the right protocol and kit size to use for each antibody you wish to label. Also see the Mix-n-Stain™ Kit Compatibility and Protocol Selection Flowchart, below.

Note that Mix-n-Stain™ Maxi 1 mg Scale Kits, Mix-n-Stain™ Fluorescent Protein Labeling Kits (R-PE, APC, PerCP, or tandem dye), Mix-n-Stain™ Enzyme Labeling Kits (AP, HRP, GOx) have different compatibility requirements and labeling protocols. Check the protocol for your kit to verify that your antibody is compatible with labeling, then choose a kit size that matches your the amount of antibody you wish to label.

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