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Can the labeling reaction tolerate the presence of BSA or gelatin?

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While labeling antibody without stabilizer proteins generally gives the best results, but antibodies with BSA or gelatin can be labeled with Mix-n-Stain™ CF® Dye Antibody Labeling Kits. The BSA or gelatin will also be labeled, but due to the high solubility of CF® dyes, the labeled BSA or gelatin is washed away during standard immunofluorescence staining. We recommend downloading the updated Product Protocol and completing the pre-labeling checklist to find the right protocol and kit size to use for each antibody you wish to label. Also see the Mix-n-Stain™ Kit Compatibility and Protocol Selection Flowchart, below.

Note that Mix-n-Stain™ Maxi 1 mg Scale Kits, Mix-n-Stain™ Fluorescent Protein Labeling Kits (R-PE, APC, PerCP, or tandem dye), Mix-n-Stain™ Enzyme Labeling Kits (AP, HRP, GOx) have different compatibility requirements and labeling protocols. Check the protocol for your kit to verify that your antibody is compatible with labeling, then choose a kit size that matches your the amount of antibody you wish to label.

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