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Is the labeling reaction compatible with ascites fluid, serum or hybridoma supernatant?

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IgG in ascites fluid can be labeled with Mix-n-Stain™ CF® Dye Antibody Labeling Kits with good results using a larger sized kit and our modified Mix-n-Stain™ protocol. Download the updated Product Protocol and complete the pre-labeling checklist to select the appropriate kit size and labeling protocol.

Mix-n-Stain™ Maxi 1 mg Scale Kits, Mix-n-Stain™ Fluorescent Protein Labeling Kits (R-PE, APC, PerCP, or tandem dye), Mix-n-Stain™ Enzyme Labeling Kits (AP, HRP, GOx) have different compatibility requirements and labeling protocols, and cannot be used to label antibodies in ascites fluid. Check the protocol for your kit to verify that your antibody is compatible with labeling, then choose a kit size that matches your the amount of antibody you wish to label.

Mix-n-Stain™ labeling does not work well for antibodies in serum or hybridoma cell culture supernatant. We recommend purifying IgG from serum or supernatant before labeling.

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