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Is the labeling reaction compatible with small molecule stabilizer/buffer components, such as sodium azide, EDTA, sugars, glycerol, DTT, 2-mercaptoethanol, glycine, and amino acids?

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For CF® dye, cyanine dye, FITC, biotin, DNP, and dig Mix-n-Stain™ kits, sodium azide, EDTA, small sugars, and <10% glycerol have no effect on the labeling. Higher levels of glycerol, or any level of DTT, 2-mercaptoethanol or free amino acids (such as glycine) should be removed using the ultrafiltration vial provided in the kit. See the Product Protocol for more information.

Note that Mix-n-Stain™ Maxi 1 mg Scale Kits, Mix-n-Stain™ Fluorescent Protein Labeling Kits (R-PE, APC, PerCP, or tandem dye), Mix-n-Stain™ Enzyme Labeling Kits (AP, HRP, GOx) have different compatibility requirements and labeling protocols. Check the protocol for your kit to verify that your antibody is compatible with labeling, then choose a kit size that matches your the amount of antibody you wish to label.

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