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I performed immunofluorescence staining with my labeled antibody, but I don’t see any signal. What should I do?

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For CF® Dye, biotin, dig, or DNP  kits, please download the updated Product Protocol and follow the Troubleshooting Checklist for step-by-step troubleshooting of Mix-n-Stain™ labeling and subsequent staining.

Note that Mix-n-Stain™ Maxi 1 mg Scale Kits, Mix-n-Stain™ Fluorescent Protein Labeling Kits (R-PE, APC, PerCP, or tandem dye), Mix-n-Stain™ Enzyme Labeling Kits (AP, HRP, GOx) have different compatibility requirements and labeling protocols. Check the protocol for your kit to verify that your antibody is compatible with labeling, then contact technical support for assistance.

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