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Agarose LE, Ultra-Pure Molecular Biology Grade

High performance agarose for nucleic acid electrophoresis (analytical or preparative) and blotting. Validated for use with Biotium’s GelRed®, GelGreen®, and DNAzure® gel stains.


  • Electrophoresis of DNA or RNA
  • Restriction enzyme mapping
  • PCR amplicon detection
  • Southern/northern blotting
  • Cloning
  • DNA band excision & purification
  • CRISPR/Cas9 mutation screening
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Product Description

Ultra-pure LE Agarose can be used for all routine electrophoresis applications. It has low electroendosmosis (EEO) which confers high electrophoretic mobility. This agarose has excellent performance for analytical or preparative nucleic acid electrophoresis and blotting. Ultra-pure LE Agarose has high strength and shows low background after staining with common nucleic acid dyes. Each lot is validated for use with Biotium’s GelRed®, GelGreen®, and DNAzure® gel stains.


  • Moisture:  ≤ 10%
  • Ash:  ≤ 0.4%
  • EEO:  0.05-0.13
  • Sulfate:  ≤ 0.1%
  • Clarity (1.5% gel):  ≤ 3 NTU
  • Gel strength (1% gel): ≥ 1200 g/cm2
  • Gel strength (1.5% gel):  ≥ 2500 g/cm2
  • Gelling temperature (1.5% agarose):  36 ± 1.5 °C
  • Melting temperature (1.5% gel): 88 ± 1.5 °C
  • DNAse/RNase activity:  None detected
  • DNA resolution ≥1000 bp: Finely resolved
  • Gel background: Very low
  • Performance in GelRed® precast dsDNA staining:  Verified
  • Performance in GelGreen® precast dsDNA staining:  Verified
  • Performance in DNAzure® visible blue dsDNA staining:  Verified

Also see our GelRed® Agarose, precoated with non-toxic, non-mutagenic GelRed® Dye, for greater safety and convenience.


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