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Agarose LE, Ultra-Pure Molecular Biology Grade

High performance agarose for nucleic acid electrophoresis (analytical or preparative) and blotting. Validated for use with Biotium’s GelRed®, GelGreen®, and DNAzure® gel stains.

Product Attributes

Storage Conditions

Store at room temperature

Specifications

  • Moisture:  ≤ 10%
  • Ash:  ≤ 0.4%
  • EEO:  0.05-0.13
  • Sulfate:  ≤ 0.1%
  • Clarity (1.5% gel):  ≤ 3 NTU
  • Gel strength (1% gel): ≥ 1200 g/cm2
  • Gel strength (1.5% gel):  ≥ 2500 g/cm2
  • Gelling temperature (1.5% agarose):  36 ± 1.5 °C
  • Melting temperature (1.5% gel): 88 ± 1.5 °C
  • DNAse/RNase activity:  None detected
  • DNA resolution ≥1000 bp: Finely resolved
  • Gel background: Very low
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

Ultra-Pure LE Agarose can be used for all routine electrophoresis applications. This agarose has excellent performance for analytical or preparative nucleic acid electrophoresis and blotting.

  • High quality, molecular biology grade agarose
  • Excellent DNA resolution with low background
  • No detectable DNase/RNase activity
  • Ideal for all routine electrophoresis and blotting applications

It has low electroendosmosis (EEO) which confers high electrophoretic mobility. Ultra-pure LE Agarose has high strength and shows low background after staining with common nucleic acid dyes. Each lot is validated for use with Biotium’s GelRed®GelGreen®, and DNAzure® gel stains.

High Quality and High Performance

Biotium’s Ultra-Pure LE Agarose has been evaluated to meet the follows specifications; moisture, ash and sulfate content, electroendosmosis, clarity, gel strength, gelling temperature, melting temperature, and DNase/RNase activity. See Sidebar or PI for details.

Also see our GelRed® Agarose, precoated with non-toxic, non-mutagenic GelRed® Dye, for greater safety and convenience.

References

  1. Infect Drug Resist, 13, 333, (2020), DOI: 10.2147/IDR.S234295
  2. J. Mar. Aquat. Sci, 5, 142, (2019), DOI: 10.24843/jmas.2019.v05.i01.p17
  3. Fungal ecology, 31, 9, (2018), DOI: 10.1016/j.funeco.2017.10.003
  4. Revista MVZ Córdoba, 23, 6778, (2018), DOI: 10.21897/rmvz.1367
  5. Parasitology, 144, 730, (2017), DOI: 10.1017/S003118201600250X
  6. J Microbiol, 10, e14378, (2017), DOI: 10.5812/jjm.14378
 

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