Support & Resources

+1

Agarose LE, Ultra-Pure Molecular Biology Grade

High performance agarose for nucleic acid electrophoresis (analytical or preparative) and blotting. Validated for use with Biotium’s GelRed®, GelGreen®, and DNAzure® gel stains.

Applications:

  • Electrophoresis of DNA or RNA
  • Restriction enzyme mapping
  • PCR amplicon detection
  • Southern/northern blotting
  • Cloning
  • DNA band excision & purification
  • CRISPR/Cas9 mutation screening
Size
Catalog #
price
Qty
25 g
100 g
500 g
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Protocols
SDS

Product Description

Ultra-pure LE Agarose can be used for all routine electrophoresis applications. It has low electroendosmosis (EEO) which confers high electrophoretic mobility. This agarose has excellent performance for analytical or preparative nucleic acid electrophoresis and blotting. Ultra-pure LE Agarose has high strength and shows low background after staining with common nucleic acid dyes. Each lot is validated for use with Biotium’s GelRed®, GelGreen®, and DNAzure® gel stains.

Specifications

  • Moisture:  ≤ 10%
  • Ash:  ≤ 0.4%
  • EEO:  0.05-0.13
  • Sulfate:  ≤ 0.1%
  • Clarity (1.5% gel):  ≤ 3 NTU
  • Gel strength (1% gel): ≥ 1200 g/cm2
  • Gel strength (1.5% gel):  ≥ 2500 g/cm2
  • Gelling temperature (1.5% agarose):  36 ± 1.5 °C
  • Melting temperature (1.5% gel): 88 ± 1.5 °C
  • DNAse/RNase activity:  None detected
  • DNA resolution ≥1000 bp: Finely resolved
  • Gel background: Very low
  • Performance in GelRed® precast dsDNA staining:  Verified
  • Performance in GelGreen® precast dsDNA staining:  Verified
  • Performance in DNAzure® visible blue dsDNA staining:  Verified

Also see our GelRed® Agarose, precoated with non-toxic, non-mutagenic GelRed® Dye, for greater safety and convenience.

 

You may also like…

    6X DNA Loading Buffer (Blue)

    6X DNA Loading Buffer (Blue) contains density agents for loading DNA samples on gels, with two blue electrophoresis tracking dyes.

    6X GelRed® Prestain Loading Buffer with Tracking Dye

    6X GelRed® Prestain Loading Buffers are DNA gel loading buffers containing GelRed® for one-step fluorescent staining and gel loading of DNA. Choose loading buffer with two blue tracking dyes or loading buffer with orange tracking dye.

    DNA Gel Extraction Kit

    Biotium's DNA Gel Extraction Kit is a silica-gel spin column based DNA extraction kit designed to purify 40 bp-40 kb DNA fragments from agarose gels in TAE or TBE buffer. Each column has a maximum binding capacity of 10 ug DNA.

    Gel-Bright™ LED Gel Illuminator

    The Gel-Bright™ LED Gel Illuminator is a non-UV lightbox for detection of fluorescently labeled nucleic acids and protein.

    GelRed® Agarose LE

    GelRed® Agarose LE is  pre-coated with GelRed® Nucleic Acid Gel Stain for simple preparation of precast gels. With no need to handle concentrated fluorescent dye while preparing your gel, GelRed® Agarose offers greater convenience and safety.

    Ready-to-Use 1 kb DNA Ladder

    1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The ladder consists of 13 bands that are generated from PCR and restriction enzyme digestion of double stranded DNA.