ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktails

The ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktails were developed for phenotyping EV samples by flow cytometry. The mixtures have been meticulously optimized for optimal brightness and signal-to-noise. The cocktails are available in blue/green/far-red or green/orange/far-red.

Skip the optimization, get results faster

The most commonly used markers for extracellular vesicle (EV) detection are the three tetraspanin proteins CD9, CD63, and CD81. Depending on the source of the EVs, each of these proteins will be present at varying levels. The ExoBrite CD9/CD63/CD81 3-Color Antibody Cocktails were designed for fast, reproducible phenotyping of an EV sample by flow cytometry. Each individual antibody in the mixture has been validated to have the same coverage as its respective ExoBrite™ Flow Antibody, ensuring accurate results.

In addition, like all ExoBrite™ Flow antibodies, the ExoBrite™ cocktails are provided in a proprietary buffer formulation for reduced antibody aggregation and brighter EV staining for optimal accuracy and signal-to-noise. The cocktails can be used on their own, for tetraspanin profiling, or combined with other antibodies of interest for more multi-target analyses.

EV antibodies you can trust

Other commercially available antibodies for tetraspanin proteins CD9, CD63, and CD81 are generally not validated for isolated EVs and may require tedious optimization for your EV prep and staining protocol. The antibodies and dye labels of ExoBrite™ Flow Antibody Conjugates were carefully selected and validated for robust detection of isolated EVs. See the table below for a full list of available ExoBrite™ Flow Antibody Conjugates. ExoBrite™ Isotype Control Flow Antibodies, which have been found to not react with any target in human cells and have the same isotype as the tetraspanin antibodies, are also available as a negative control.

Accelerate your EV research with ExoBrite™ EV stains

For general EV staining, Biotium’s ExoBrite™ True EV Membrane Stains offer unparalleled coverage of EVs in a sample and address issues of dye aggregation often seen with PKH and other common membrane dyes. Biotium also offers optimized ExoBrite™ EV Surface stains conjugated to cholera toxin B (CTB), wheat germ agglutinin (WGA), and Annexin V. These stains are specially formulated for bright and specific detection of isolated EVs by flow cytometry. These ExoBrite™ EV Surface stains may also be combined with antibody staining, for multi-parameter analysis.

Biotium also offers conjugated ExoBrite™ Western Antibodies against CD9, CD63, and CD81 designed for optimal detection in EV extracts by fluorescent western blot.

Note: In our testing, we have found that ExoBrite™ 490/515 dye may bind to streptavidin coated surfaces or beads if free biotin binding sites are not blocked. We recommend performing a biotin blocking step after binding your biotinylated capture antibody to streptavidin beads or surfaces when using ExoBrite™ 490/515 conjugates. Alternatively, consider using a different ExoBrite™ dye for staining EVs captured on streptavidin beads or surfaces.

ExoBrite™ Antibody Cocktails

ExoBrite™ Flow Antibody Conjugates