Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

Goat Anti-Mouse IgG2a

CF™ dye goat anti-mouse IgG2a isotype-specific secondary antibody conjugates are labeled with our exceptionally bright, photostable CF™ dyes. The conjugates are prepared from affinity-purified antibodies that react with Fc portion of the heavy chain of mouse IgG2a.

Placeholder

Secondary antibody formats:
Liquid format:

  • CF™ dye and biotin conjugates: 2 mg/mL in PBS, 50% glycerol, 2 mg/ml BSA, 0.05% sodium azide
  • HRP conjugates: 1 mg/mL in PBS, 50% glycerol, 15 mg/ml BSA
  • Alkaline phosphatase conjugates: 1 mg/mL in PBS, 40% glycerol, 5 mg/ml BSA, 0.05% sodium azide
  • R-PE and APC conjugates: 0.5 mg/mL in PBS, 2 mg/ml
    BSA, 0.05% sodium azide

Lyophilized format (after reconstitution):

  • CF™ dye and biotin conjugates: 2 mg/mL in PBS, 15 mg/mL BSA, 20 mg/mL trehalose
  • HRP conjugates: 1 mg/mL PBS, 10 mg/mL trehalose
Catalog #

20255, 20258-1, 20259-1, 20260-1, 20261-1, 20262-1, 20263-1, 20264-1, 20326-1, 20381-250uL, 20381-50uL, 20472-250uL, 20257-1, 20256-1, 20256, 20257, 20258, 20259, 20260, 20261, 20262, 20263, 20264, 20326, 20255-1, - 20472-50uLView allHide

Size

50 uL, 250 uL, 0.25 mL

Conjugation

Biotin, CF350, CF405S, CF488A, CF543, CF555, CF568, CF594, CF633, CF640R, CF647, CF680, CF770

Antibody type

Secondary

Species reactivity

Mouse

Host species

Goat

Reactivity (target)

Mouse, Mouse IgG2a

Cross adsorption

Bovine, Rabbit, Human

Applications

Immunofluorescence, Immunohistology (formalin), Western, Immunohistology (frozen)

Conjugation
Size
Catalog #
price
Qty
50 uL
250 uL
0.25 mL
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping & Tax (CA only) not included in price. Learn more

Taxes: Tax is assessed in the state of California and is calculated during checkout based on your shipping information. Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

CF™ dye goat anti-mouse IgG2a isotype-specific secondary antibody conjugates are labeled with our exceptionally bright, photostable CF™ dyes and other labels. The conjugates are prepared from affinity-purified antibodies that react with Fc portion of the heavy chain of mouse IgG2a. To minimize cross reactivity, the antibodies are cross-adsorbed against other mouse IgG subclasses (IgG1, IgG2b, IgG3), and human, bovine and rabbit serum proteins. CF™ dyes offer exceptional brightness and photostability. See the CF™ Dye Selection Guide under Downloads for more information.

Note: Conjugates of blue fluorescent dyes like CF™350 and CF™405S are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Goat Anti-Mouse IgG2a: Product & SKU Summary

ProductConjugationEx/EmSizeSKUPurchase
Biotin Goat Anti-Mouse IgG2aBiotinN/A250 μL20472-250uLPurchase 20472-250uL
Biotin Goat Anti-Mouse IgG2aBiotinN/A50 μL20472-50uLPurchase 20472-50uL
CF350 Goat Anti-Mouse IgG2aCF350347/448 nm0.25 mL20255Purchase 20255
CF350 Goat Anti-Mouse IgG2aCF350347/448 nm50 μL20255-1Purchase 20255-1
CF405S Goat Anti-Mouse IgG2aCF405S404/431 nm0.25 mL20381-250uLPurchase 20381-250uL
CF405S Goat Anti-Mouse IgG2aCF405S404/431 nm50 μL20381-50uLPurchase 20381-50uL
CF488A Goat Anti-Mouse IgG2aCF488A490/515 nm0.25 mL20256Purchase 20256
CF488A Goat Anti-Mouse IgG2aCF488A490/515 nm50 μL20256-1Purchase 20256-1
CF543 Goat Anti-Mouse IgG2aCF543541/560 nm0.25 mL20326Purchase 20326
CF543 Goat Anti-Mouse IgG2aCF543541/560 nm50 μL20326-1Purchase 20326-1
CF555 Goat Anti-Mouse IgG2aCF555555/565 nm0.25 mL20257Purchase 20257
CF555 Goat Anti-Mouse IgG2aCF555555/565 nm50 μL20257-1Purchase 20257-1
CF568 Goat Anti-Mouse IgG2aCF568562/583 nm0.25 mL20258Purchase 20258
CF568 Goat Anti-Mouse IgG2aCF568562/583 nm50 μL20258-1Purchase 20258-1
CF594 Goat Anti-Mouse IgG2aCF594593/614 nm0.25 mL20259Purchase 20259
CF594 Goat Anti-Mouse IgG2aCF594593/614 nm50 μL20259-1Purchase 20259-1
CF633 Goat Anti-Mouse IgG2aCF633630/650 nm0.25 mL20260Purchase 20260
CF633 Goat Anti-Mouse IgG2aCF633630/650 nm50 μL20260-1Purchase 20260-1
CF640R Goat Anti-Mouse IgG2aCF640R642/662 nm0.25 mL20261Purchase 20261
CF640R Goat Anti-Mouse IgG2aCF640R642/662 nm50 μL20261-1Purchase 20261-1
CF647 Goat Anti-Mouse IgG2aCF647650/665 nm0.25 mL20262Purchase 20262
CF647 Goat Anti-Mouse IgG2aCF647650/665 nm50 μL20262-1Purchase 20262-1
CF680 Goat Anti-Mouse IgG2aCF680681/698 nm0.25 mL20263Purchase 20263
CF680 Goat Anti-Mouse IgG2aCF680681/698 nm50 μL20263-1Purchase 20263-1
CF770 Goat Anti-Mouse IgG2aCF770770/797 nm0.25 mL20264Purchase 20264
CF770 Goat Anti-Mouse IgG2aCF770770/797 nm50 μL20264-1Purchase 20264-1

References

Download a list of CF dye references.

 

You may also like…

Support & Faq

Product shelf life, solubility, shipping and storage, and stability

Bioscience kits
The guaranteed shelf life from date of receipt for bioscience kits is listed on the product information sheet. Some kits have an expiration date printed on the kit box label, this is the guaranteed shelf life date calculated from the day that the product shipped from our facility. Kits often are functional for significantly longer than the guaranteed shelf life. If you have an older kit in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the kit still works for your application before processing a large number of samples or precious samples.

Antibodies and other conjugates
The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. If you have an older conjugate in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.

For lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates).

Chemicals, dyes, and gel stains
Biotium guarantees the stability of chemicals, dyes, and gel stains for at least a year from the date you receive the product. However, the majority of these products are highly stable for many years, as long as they are stored as recommended. Storage conditions can be found on the product information sheet or product safety and data sheet, material safety data sheet, and on the product label. Fluorescent compounds should be protected from light for long term storage.

If you have a Biotium compound that has been in storage for longer than one year that you wish to use, we recommend performing a small scale positive control experiment to confirm that the compound still works for your application before processing a large number of samples or precious samples.

Expiration date based on date of manufacture (DOM)
If your institution requires you to document expiration date based on date of manufacture for reagents, please contact techsupport@biotium.com for assistance.

Chemical products with special stability considerations:

Esters

Ester compounds include the following:
• Succinimidyl esters (SE, also known as NHS esters), such as our amine-reactive dyes
• Acetoxymethyl esters (AM esters) such as our membrane-permeable ion indicator dyes
• Diacetate-modified dyes, like ViaFluor™ 405, CFDA, and CFDA-SE cell viability/cell proliferation dyes

Ester dyes are stable in solid form as long as they are protected from light and moisture. Esters are not stable in aqueous solution. Concentrated stock solutions should be prepared in anhydrous DMSO (see Biotium catalog no. 90082). Stock solutions in anhydrous DMSO can be stored desiccated at -20°C for one month or longer. Esters should be diluted in aqueous solution immediately before use. Succinimidyl esters (SE) should be dissolved in a solution that is free of amine-containing compounds like Tris, glycine, or protein, which will react with the SE functional group. AM esters and diacetate compounds should be dissolved in a solution that is free of serum, because serum could contain esterases that would hydrolyze the compound.

A note on CF™ dye succinimidyl ester stability
Succinimidyl esters are generally susceptible to hydrolysis, which can result in lower labeling efficiency. Heavily sulfonated dyes, such as the Alexa Fluor® dyes, DyLight® dyes and IRDyes® are particularly hygroscopic, worsening the hydrolysis problem. For example, the percent of active Alexa Fluor® 488 succinimidyl ester (SE) could be well below 50% by the time of application (according to the manufacturer’s product datasheet). In a number of Alexa Fluor® SE reactive dyes, the SE group is derived from an aromatic carboxylic acid, while in all of Biotium’s CF™ dyes the SE group is prepared from an aliphatic carboxylic acid. This structural difference reduces the susceptibility of CF™ dye SE reactive groups to hydrolysis, resulting in relatively stable reactive dyes with consistently higher labeling efficiency compared to other SE derivatives of other fluorescent dyes.

Maleimides, MTS and thiosulfate dyes
Like the succinimidyl ester dyes, these dyes are also susceptible to hydrolysis, although generally to a much lower degree. Thus, for long term storage, anhydrous DMSO is recommended for making stock solutions.

Other reactive dyes
Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react with the dye’s functional group. See the product information sheets for specific reactive dyes more information.

Coelenterazines and D-luciferin

Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. Propylene glycol also can be used as a solvent to minimize evaporation. If the solvent evaporates, the coelenterazine will still be present in the vial, so note the volume in the vial prior to storage so that you can adjust the solvent volume to correct for evaporation if needed. Prepare working solutions in aqueous buffers immediately before use. Coelenterazines are stable for up to five hours in aqueous solution.

Aquaphile™ coelenterazines are water soluble formulations of coelenterazines. They are stable in solid form when stored as recommended. Aquaphile™ coelenterazines should be dissolved in aqueous solution immediately before use. They are stable for up to five hours in aqueous solution.

Note that coelenterazines are predominantly yellow solids, but may contain dark red or brown flecks. This does not affect product stability or performance. If your coelenterazine is uniformly brown, then it is oxidized and needs to be replaced.

D-luciferin is stable in solid form and as a concentrated stock solution when stored as recommended; it is not stable at dilute working concentrations in aqueous solution. Prepare concentrated D-luciferin stock solutions (typically 1-100 mg/mL) in water, and store in aliquots at -20°C or below for six months or longer. Prepare working solutions immediately before use.

Category: Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView™ Caspase 3 Enzyme Substrates, Luciferase Assays, CF™ Dyes, Product shelf life, solubility, shipping and storage, and stability

← FAQs

Many of our solid compounds are packaged by lyophilization, in which case they usually do not appear as fluffy powders, but form a film or coating on the sides of the vial. Simply add the appropriate volume of the recommended solvent to the vial to make the desired concentration stock solution, and swirl or gently vortex to mix. Make sure the solvent comes in contact with the inside walls of the vial to fully recover the product.

Category: Product shelf life, solubility, shipping and storage, and stability

← FAQs

Most of our products are stable at room temperature for many days, but we recommend storage at 4°C or -20°C to prolong shelf life. In the case of many of our aqueous dye solutions, the compounds are very stable at room temperature, but we recommend cold storage to prevent the growth of mold or other microbes over time. Therefore, to save on shipping costs, products with recommended storage at 4°C or -20°C may ship at ambient temperature without affecting product performance. When you receive the product, place it under the recommended storage conditions.

Category: Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView™ Caspase 3 Enzyme Substrates, Luciferase Assays, CF™ Dyes, Product shelf life, solubility, shipping and storage, and stability

← FAQs

← FAQs

Ask a question

Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.