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Goat Anti-Mouse IgG2a (γ2a)

Goat anti-mouse IgG2a isotype-specific secondary antibody labeled with our superior CF® Dyes and biotin.

Product Attributes

Antibody type

Secondary

Clonality

Polyclonal

Host species

Goat

Antibody reactivity (target)

Mouse IgG2a (γ2a)

Species reactivity

Mouse

Cross adsorption

Bovine, Human, Rabbit

Concentration

2 mg/mL

Antibody/conjugate formulation

Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide, Lyophilized: PBS/15 mg/mL BSA/20 mg/mL trehalose after reconstitution

Secondary/tag antibody applications

ELISA, Flow cytometry, IHC, IF (cells or tissue sections), Western blot

Conjugation
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250 uL
0.25 mL
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Product Description

This is a goat anti-mouse IgG2a isotype-specific secondary antibody labeled with our bright and photostable CF® Dyes and biotin. The conjugates are prepared from affinity-purified antibodies that react with Fc portion of the heavy chain of mouse IgG2a. To minimize cross reactivity, the antibodies are cross-adsorbed against other mouse IgG subclasses (IgG1, IgG2b, IgG3), and human, bovine and rabbit serum proteins.

  • Cross-adsorbed for specific staining with minimal background
  • Available in 13 bright and photostable CF® Dyes
  • Biotin conjugate also available
  • Suitable for western, immunofluorescence, and immunohistology

Note: Conjugates of blue fluorescent dyes like CF®350 and CF®405S are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Goat Anti-Mouse IgG2a

ConjugationEx/EmSizeCatalog No.
CF®350347/448 nm50 uL (100 ug)20255-1
0.25 mL (500 ug)20255
CF®405S404/431 nm50 uL (100 ug)20381-50uL
0.25 mL (500 ug)20381-250uL
CF®488A490/515 nm50 uL (100 ug)20256-1
0.25 mL (500 ug)20256
CF®543541/560 nm50 uL (100 ug)20326-1
0.25 mL (500 ug)20326
CF®555555/565 nm50 uL (100 ug)20257-1
0.25 mL (500 ug)20257
CF®568562/583 nm50 uL (100 ug)20258-1
0.25 mL (500 ug)20258
CF®594593/614 nm50 uL (100 ug)20259-1
0.25 mL (500 ug)20259
CF®633630/650 nm50 uL (100 ug)20260-1
0.25 mL (500 ug)20260
CF®640R642/662 nm50 uL (100 ug)20261-1
0.25 mL (500 ug)20261
CF®647650/665 nm50 uL (100 ug)20262-1
0.25 mL (500 ug)20262
CF®680681/698 nm50 uL (100 ug)20263-1
0.25 mL (500 ug)20263
CF®680R680/701 nm50 uL (100 ug)20842-50uL
0.25 mL (500 ug)20842-250uL
CF®770770/797 nm50 uL (100 ug)20264-1
0.25 mL (500 ug)20264
BiotinN/A50 uL (100 ug)20472-50uL
0.25 mL (500 ug)20472-250uL

View our full selection of bright and specific Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of curated CF® Dye references.

 

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