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Goat Anti-Mouse IgG2b

CF™ dye goat anti-mouse IgG2b isotype-specific secondary antibody conjugates are labeled with our exceptionally bright, photostable CF™ dyes. The conjugates are prepared from affinity-purified antibodies that react with Fc portion of the heavy chain of mouse IgG2b. To minimize cross reactivity, the antibodies are cross-adsorbed against other mouse IgG subclasses (IgG1, IgG2a, IgG3), and human, bovine and rabbit serum proteins.

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Secondary antibody formats:
Liquid format:

  • CF™ dye and biotin conjugates: 2 mg/mL in PBS, 50% glycerol, 2 mg/ml BSA, 0.05% sodium azide
  • HRP conjugates: 1 mg/mL in PBS, 50% glycerol, 15 mg/ml BSA
  • Alkaline phosphatase conjugates: 1 mg/mL in PBS, 40% glycerol, 5 mg/ml BSA, 0.05% sodium azide
  • R-PE and APC conjugates: 0.5 mg/mL in PBS, 2 mg/ml
    BSA, 0.05% sodium azide

Lyophilized format (after reconstitution):

  • CF™ dye and biotin conjugates: 2 mg/mL in PBS, 15 mg/mL BSA, 20 mg/mL trehalose
  • HRP conjugates: 1 mg/mL PBS, 10 mg/mL trehalose
Catalog #

20265, 20269-1, 20270-1, 20271-1, 20272-1, 20273-1, 20274-1, 20327-1, 20382-250uL, 20382-50uL, 20430-250uL, 20430-50uL, 20473-250uL, 20268-1, 20267-1, 20266, 20267, 20268, 20269, 20270, 20271, 20272, 20273, 20274, 20327, 20265-1, 20266-1, - 20473-50uLView allHide

Size

50 uL, 250 uL, 0.25 mL

Conjugation

Biotin, CF350, CF405S, CF488A, CF543, CF555, CF568, CF594, CF633, CF640R, CF647, CF680, CF750, CF770

Antibody type

Secondary

Species reactivity

Mouse

Host species

Goat

Reactivity (target)

Mouse, Mouse IgG2b

Cross adsorption

Bovine, Rabbit, Human

Applications

Immunofluorescence, Immunohistology (formalin), Western, Immunohistology (frozen)

Conjugation
Size
Catalog #
price
Qty
50 uL
250 uL
0.25 mL
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Product Description

CF™ dye goat anti-mouse IgG2b isotype-specific secondary antibody conjugates are labeled with our exceptionally bright, photostable CF™ dyes and other labels. The conjugates are prepared from affinity-purified antibodies that react with Fc portion of the heavy chain of mouse IgG2b. To minimize cross reactivity, the antibodies are cross-adsorbed against other mouse IgG subclasses (IgG1, IgG2a, IgG3), and human, bovine and rabbit serum proteins. CF™ dyes offer exceptional brightness and photostability. See the CF™ Dye Selection Guide under Downloads for more information.

Goat Anti-Mouse IgG2b: Product & SKU Summary

ProductConjugationEx/EmSizeSKUPurchase
Biotin Goat Anti-Mouse IgG2bBiotinN/A250 μL20473-250uLPurchase 20473-250uL
Biotin Goat Anti-Mouse IgG2bBiotinN/A50 μL20473-50uLPurchase 20473-50uL
CF350 Goat Anti-Mouse IgG2bCF350347/448 nm0.25 mL20265Purchase 20265
CF350 Goat Anti-Mouse IgG2bCF350347/448 nm50 μL20265-1Purchase 20265-1
CF405S Goat Anti-Mouse IgG2bCF405S404/431 nm0.25 mL20382-250uLPurchase 20382-250uL
CF405S Goat Anti-Mouse IgG2bCF405S404/431 nm50 μL20382-50uLPurchase 20382-50uL
CF488A Goat Anti-Mouse IgG2bCF488A490/515 nm0.25 mL20266Purchase 20266
CF488A Goat Anti-Mouse IgG2bCF488A490/515 nm50 μL20266-1Purchase 20266-1
CF543 Goat Anti-Mouse IgG2bCF543541/560 nm0.25 mL20327Purchase 20327
CF543 Goat Anti-Mouse IgG2bCF543541/560 nm50 μL20327-1Purchase 20327-1
CF555 Goat Anti-Mouse IgG2bCF555555/565 nm0.25 mL20267Purchase 20267
CF555 Goat Anti-Mouse IgG2bCF555555/565 nm50 μL20267-1Purchase 20267-1
CF568 Goat Anti-Mouse IgG2bCF568562/583 nm0.25 mL20268Purchase 20268
CF568 Goat Anti-Mouse IgG2bCF568562/583 nm50 μL20268-1Purchase 20268-1
CF594 Goat Anti-Mouse IgG2bCF594593/614 nm0.25 mL20269Purchase 20269
CF594 Goat Anti-Mouse IgG2bCF594593/614 nm50 μL20269-1Purchase 20269-1
CF633 Goat Anti-Mouse IgG2bCF633630/650 nm0.25 mL20270Purchase 20270
CF633 Goat Anti-Mouse IgG2bCF633630/650 nm50 μL20270-1Purchase 20270-1
CF640R Goat Anti-Mouse IgG2bCF640R642/662 nm0.25 mL20271Purchase 20271
CF640R Goat Anti-Mouse IgG2bCF640R642/662 nm50 μL20271-1Purchase 20271-1
CF647 Goat Anti-Mouse IgG2bCF647650/665 nm0.25 mL20272Purchase 20272
CF647 Goat Anti-Mouse IgG2bCF647650/665 nm50 μL20272-1Purchase 20272-1
CF680 Goat Anti-Mouse IgG2bCF680681/698 nm0.25 mL20273Purchase 20273
CF680 Goat Anti-Mouse IgG2bCF680681/698 nm50 μL20273-1Purchase 20273-1
CF750 Goat Anti-Mouse IgG2bCF750755/777 nm250 μL20430-250uLPurchase 20430-250uL
CF750 Goat Anti-Mouse IgG2bCF750755/777 nm50 μL20430-50uLPurchase 20430-50uL
CF770 Goat Anti-Mouse IgG2bCF770770/797 nm0.25 mL20274Purchase 20274
CF770 Goat Anti-Mouse IgG2bCF770770/797 nm50 μL20274-1Purchase 20274-1

References

Download a list of CF dye references.

 

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Support & Faq

Product shelf life, solubility, shipping and storage, and stability

Bioscience kits
The guaranteed shelf life from date of receipt for bioscience kits is listed on the product information sheet. Some kits have an expiration date printed on the kit box label, this is the guaranteed shelf life date calculated from the day that the product shipped from our facility. Kits often are functional for significantly longer than the guaranteed shelf life. If you have an older kit in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the kit still works for your application before processing a large number of samples or precious samples.

Antibodies and other conjugates
The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. If you have an older conjugate in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.

For lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates).

Chemicals, dyes, and gel stains
Biotium guarantees the stability of chemicals, dyes, and gel stains for at least a year from the date you receive the product. However, the majority of these products are highly stable for many years, as long as they are stored as recommended. Storage conditions can be found on the product information sheet or product safety and data sheet, material safety data sheet, and on the product label. Fluorescent compounds should be protected from light for long term storage.

If you have a Biotium compound that has been in storage for longer than one year that you wish to use, we recommend performing a small scale positive control experiment to confirm that the compound still works for your application before processing a large number of samples or precious samples.

Expiration date based on date of manufacture (DOM)
If your institution requires you to document expiration date based on date of manufacture for reagents, please contact techsupport@biotium.com for assistance.

Chemical products with special stability considerations:

Esters

Ester compounds include the following:
• Succinimidyl esters (SE, also known as NHS esters), such as our amine-reactive dyes
• Acetoxymethyl esters (AM esters) such as our membrane-permeable ion indicator dyes
• Diacetate-modified dyes, like ViaFluor™ 405, CFDA, and CFDA-SE cell viability/cell proliferation dyes

Ester dyes are stable in solid form as long as they are protected from light and moisture. Esters are not stable in aqueous solution. Concentrated stock solutions should be prepared in anhydrous DMSO (see Biotium catalog no. 90082). Stock solutions in anhydrous DMSO can be stored desiccated at -20°C for one month or longer. Esters should be diluted in aqueous solution immediately before use. Succinimidyl esters (SE) should be dissolved in a solution that is free of amine-containing compounds like Tris, glycine, or protein, which will react with the SE functional group. AM esters and diacetate compounds should be dissolved in a solution that is free of serum, because serum could contain esterases that would hydrolyze the compound.

A note on CF™ dye succinimidyl ester stability
Succinimidyl esters are generally susceptible to hydrolysis, which can result in lower labeling efficiency. Heavily sulfonated dyes, such as the Alexa Fluor® dyes, DyLight® dyes and IRDyes® are particularly hygroscopic, worsening the hydrolysis problem. For example, the percent of active Alexa Fluor® 488 succinimidyl ester (SE) could be well below 50% by the time of application (according to the manufacturer’s product datasheet). In a number of Alexa Fluor® SE reactive dyes, the SE group is derived from an aromatic carboxylic acid, while in all of Biotium’s CF™ dyes the SE group is prepared from an aliphatic carboxylic acid. This structural difference reduces the susceptibility of CF™ dye SE reactive groups to hydrolysis, resulting in relatively stable reactive dyes with consistently higher labeling efficiency compared to other SE derivatives of other fluorescent dyes.

Maleimides, MTS and thiosulfate dyes
Like the succinimidyl ester dyes, these dyes are also susceptible to hydrolysis, although generally to a much lower degree. Thus, for long term storage, anhydrous DMSO is recommended for making stock solutions.

Other reactive dyes
Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react with the dye’s functional group. See the product information sheets for specific reactive dyes more information.

Coelenterazines and D-luciferin

Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. Propylene glycol also can be used as a solvent to minimize evaporation. If the solvent evaporates, the coelenterazine will still be present in the vial, so note the volume in the vial prior to storage so that you can adjust the solvent volume to correct for evaporation if needed. Prepare working solutions in aqueous buffers immediately before use. Coelenterazines are stable for up to five hours in aqueous solution.

Aquaphile™ coelenterazines are water soluble formulations of coelenterazines. They are stable in solid form when stored as recommended. Aquaphile™ coelenterazines should be dissolved in aqueous solution immediately before use. They are stable for up to five hours in aqueous solution.

Note that coelenterazines are predominantly yellow solids, but may contain dark red or brown flecks. This does not affect product stability or performance. If your coelenterazine is uniformly brown, then it is oxidized and needs to be replaced.

D-luciferin is stable in solid form and as a concentrated stock solution when stored as recommended; it is not stable at dilute working concentrations in aqueous solution. Prepare concentrated D-luciferin stock solutions (typically 1-100 mg/mL) in water, and store in aliquots at -20°C or below for six months or longer. Prepare working solutions immediately before use.

Category: Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView™ Caspase 3 Enzyme Substrates, Luciferase Assays, CF™ Dyes, Product shelf life, solubility, shipping and storage, and stability

← FAQs

Many of our solid compounds are packaged by lyophilization, in which case they usually do not appear as fluffy powders, but form a film or coating on the sides of the vial. Simply add the appropriate volume of the recommended solvent to the vial to make the desired concentration stock solution, and swirl or gently vortex to mix. Make sure the solvent comes in contact with the inside walls of the vial to fully recover the product.

Category: Product shelf life, solubility, shipping and storage, and stability

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Most of our products are stable at room temperature for many days, but we recommend storage at 4°C or -20°C to prolong shelf life. In the case of many of our aqueous dye solutions, the compounds are very stable at room temperature, but we recommend cold storage to prevent the growth of mold or other microbes over time. Therefore, to save on shipping costs, products with recommended storage at 4°C or -20°C may ship at ambient temperature without affecting product performance. When you receive the product, place it under the recommended storage conditions.

Category: Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView™ Caspase 3 Enzyme Substrates, Luciferase Assays, CF™ Dyes, Product shelf life, solubility, shipping and storage, and stability

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.