JC-1 (chloride salt)
JC-1 (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine chloride) is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion.JC-1 monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential.
Wishlist updated! View wishlist
Shipping, Handling & Tax (CA only) not included in price.
Learn more
Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.
SPECIAL - Order 5 or more and receive 10% off.
Request a Bulk QuoteProduct Description
JC-1 (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine chloride) is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion.JC-1 monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential. The monomer JC-1 has green fluorescence (λ Em = 527 nm),while the J-aggregates have red fluorescence (λ Em = 590nm). Therefore, it is possible to use the fluorescence ratio technique to study mitochondrial membrane potentials. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green. It has also been used in high throughput drug screening applications.
JC-1, iodide (#70014) is the original salt form of the dye. Biotium developed JC-1 chloride salt for applications where iodide ion is not desired. JC-1 chloride may also have the advantage of being slightly more water soluble than the iodide salt.
- ε (MeOH) = 190,000
- Red solid soluble in DMSO
- Store at 4°C and protect from light, especially in solution
- C25H27Cl5N4
- MW: 561.5
References
1. PNAS 88, 3671 (1991).
2. Biochemistry 30, 4480 (1991).