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JC-1 (chloride salt)

JC-1 (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine chloride) is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion.JC-1 monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential.

Product Attributes

Probe cellular localization

Mitochondria

For live or fixed cells

For live/intact cells

Assay type/options

No-wash staining, Real-time imaging

Cell permeability

Membrane permeant

Apoptosis/viability marker

Mitochondrial potential

Potential dependence

Mitochondrial potential-dependent

Indicator type

Ratiometric

Colors

Green/Red

Excitation/Emission

510/527 nm (monomer); 585/590 nm (aggregate)

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Product Description

JC-1 (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine chloride) is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion.JC-1 monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential. The monomer JC-1 has green fluorescence (λ Em = 527 nm),while the J-aggregates have red fluorescence (λ Em = 590nm). Therefore, it is possible to use the fluorescence ratio technique to study mitochondrial membrane potentials. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green. It has also been used in high throughput drug screening applications.

JC-1, iodide (#70014) is the original salt form of the dye. Biotium developed JC-1 chloride salt for applications where iodide ion is not desired. JC-1 chloride may also have the advantage of being slightly more water soluble than the iodide salt.

  • λExEm 510/527 nm (monomer); 585/590 nm (aggregate)
  • ε (MeOH) = 190,000
  • Red solid soluble in DMSO
  • Store at 4°C and protect from light, especially in solution
  • C25H27Cl5N4
  • MW: 561.5

 

References

1. PNAS 88, 3671 (1991).

2. Biochemistry 30, 4480 (1991).

 

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