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MitoView™ Fix 640

Far-red, mitochondrial stain with excellent tolerance for fixation and permeabilization after staining.

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Product Description

MitoView™ Fix 640 is far-red mitochondrial stain. The cell membrane-permeant dye rapidly accumulates in the mitochondria of live cells for no-wash, live cell staining. The dye has a reactive group that can covalently attach to proteins inside the mitochondria, so that staining is well-retained after fixation, permeabilization, and subsequent immunofluorescence staining.

  • Stain mitochondria in live cells for subsequent fixation
  • Well-retained after PFA or MeOH fixation, permeabilization, and IF staining
  • Bright, photostable, and non-toxic for long-term live cell imaging
  • Cells retain specific staining in culture for days
  • Far-red fluorescence for the Cy®5 channel (Ex/Em 648/670 nm)
  • Better specificity after fixation compared to MitoTracker® Deep-Red

MitoView™ Fix is not recommended for staining cells that are already fixed, because staining is non-specific in fixed cells. MitoView™ Green dye can be used for fixed cell staining, but for best results with fixed cells, we recommend using one of our mitochondrial marker antibody conjugates.

Note: While the localization of MitoView Fix 640 does depend on mitochondrial polarization, only MitoView 633 fluorescence signal can be used to measure changes in mitochondrial potential. All other MitoView Dyes will still retain fluorescence after depolarization or fixation.

MitoView™ Dyes

ProductEx/EmDetection channelPotentiometric?SizeCat. No.
MitoView™ 405398/440 nmDAPINo†50 ug70070-T
20x50 ug70070
MitoView™ Green490/523 nmFITC, GFPNo50 ug70054-T
20x50 ug70054
MitoView™ 633622/648 nm*Cy®5, APC*Yes50 ug70055-T
20x50 ug70055
MitoView™ Fix 640648/670 nmCy®5, APCNo‡50 ug70082-50ug
10x50 ug70082
MitoView™ 650644/670 nmCy®5, APCNo50 ug70075-50ug
20x50 ug70075
MitoView™ 720720/758 nm**Cy®5, Cy®7**No†50 ug70068-T
20x50 ug70068
* MitoView™ 633 also has red fluorescence in the Cy®3 channel and is not recommended for use with other red dyes.
**While optimal for Cy®7 settings, MitoView™ 720 is bright enough to be imaged in the Cy®5 channel.
† Dyes localize to the cytoplasm after mitochondrial depolarization, but still retain fluorescence.
‡ Reacts covalently with mitochondrial proteins for staining that is retained after fixation and permeabilization.

See our full selection of Cellular Stains for mitochondria, nucleus, cell surface, lysosomes, and other organelles.

Cy Dye is a registered trademark of Cytiva; MitoTracker is a registered trademark of Thermo Fisher Scientific.
 

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70082