MitoView™ Fix 640
Far-red, mitochondrial stain with excellent tolerance for fixation and permeabilization after staining.
Please fill in the inquiry form and we will contact you shortly.
Wishlist updated! View wishlist
Product Description
MitoView™ Fix 640 is far-red mitochondrial stain. The cell membrane-permeant dye rapidly accumulates in the mitochondria of live cells for no-wash, live cell staining. The dye has a reactive group that can covalently attach to proteins inside the mitochondria, so that staining is well-retained after fixation, permeabilization, and subsequent immunofluorescence staining.
- Stain mitochondria in live cells for subsequent fixation
- Well-retained after PFA or MeOH fixation, permeabilization, and IF staining
- Bright, photostable, and non-toxic for long-term live cell imaging
- Cells retain specific staining in culture for days
- Far-red fluorescence for the Cy®5 channel (Ex/Em 648/670 nm)
- Better specificity after fixation compared to MitoTracker® Deep-Red
MitoView™ Fix is not recommended for staining cells that are already fixed, because staining is non-specific in fixed cells. MitoView™ Green dye can be used for fixed cell staining, but for best results with fixed cells, we recommend using one of our mitochondrial marker antibody conjugates.
Note: While the localization of MitoView™ Fix 640 does depend on mitochondrial polarization, only MitoView™ 633 fluorescence signal can be used to measure changes in mitochondrial potential. All other MitoView™ Dyes will still retain fluorescence after depolarization or fixation.
MitoView™ Dyes
Product | Ex/Em | Detection channel | Potentiometric? | Size | Cat. No. |
---|---|---|---|---|---|
MitoView™ 405 | 398/440 nm | DAPI | No† | 50 ug | 70070-T |
20x50 ug | 70070 | ||||
MitoView™ Green | 490/523 nm | FITC, GFP | No | 50 ug | 70054-T |
20x50 ug | 70054 | ||||
MitoView™ 633 | 622/648 nm* | Cy®5, APC* | Yes | 50 ug | 70055-T |
20x50 ug | 70055 | ||||
MitoView™ Fix 640 | 648/670 nm | Cy®5, APC | No‡ | 50 ug | 70082-50ug |
10x50 ug | 70082 | ||||
MitoView™ 650 | 644/670 nm | Cy®5, APC | No | 50 ug | 70075-50ug |
20x50 ug | 70075 | ||||
MitoView™ 720 | 720/758 nm** | Cy®5, Cy®7** | No† | 50 ug | 70068-T |
20x50 ug | 70068 |
**While optimal for Cy®7 settings, MitoView™ 720 is bright enough to be imaged in the Cy®5 channel.
† Dyes localize to the cytoplasm after mitochondrial depolarization, but still retain fluorescence.
‡ Reacts covalently with mitochondrial proteins for staining that is retained after fixation and permeabilization.
See our full selection of Cellular Stains for mitochondria, nucleus, cell surface, lysosomes, and other organelles.