Monoclonal anti CD16 (C16/1045)

Price:

from

$120.00

Catalog #: BNUB1045

Selection:

Multiple sizes

Category: Primary Antibodies.

>>> Order 5 or more and receive 10% off.

Description
References
More Info
Downloads
Shipping/shelf life

Product Description

It recognizes CD16 (FcγRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80kDa. Two similar genes represent CD16, CD16A (FcγRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (FcγRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

Knapp W. et al. (eds) Leukocyte Typing IV, Oxford University Press, Oxford, 1989. | Lanier LL et al. Functional properties of a unique subset of cytotoxic CD3+ T lymphocytes that express Fc receptors for IgG (CD16/Leu-11 antigen). J Exp Med 1985, 162(6):2089-2106

Additional Information

Conjugation	Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP
Size	50 uL, 100 uL, 250 uL, 500 uL
Supplied As	Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA
Antibody type	Primary
Host species	Mouse
Reactivity (target)	CD16 (C16/1045)
Clonality	Monoclonal
Isotype	IgM, kappa
Clone	C16/1045
Molecular weight	50-80 kDa
Synonyms
Entrez gene ID	2214
SwissProt	P08637
Unigene	372679
Immunogen	Recombinant human CD16 protein
Species reactivity	Human
Applications	Flow cytometry, Immunofluorescence
Cellular localization	Cell surface
Application notes	Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user
Positive control	K-562 or U-937 cells. Lymph nodes and tonsils.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life