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CD16 / Fc Gamma Receptor III Monoclonal Mouse Antibody (CB16)

This antibody recognizes CD16 (FcgammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa.

Product Attributes

Antibody number

0038

Reactivity (target)

CD16, Fc gamma receptor III

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

CB16

Isotype

IgM

Molecular weight

50-80 kDa

Synonyms

CD162; CLA; Cutaneous lymphocyte associated antigen; P-selectin glycoprotein ligand 1; PSGL1; Selectin P ligand; SELPLG

Human gene symbol

FCGR3A

Entrez gene ID

2214

SwissProt

P08637

Unigene

372679

Immunogen

Human CD16

Cellular localization

Membrane/cell surface

Species reactivity

Human

Applications

Immunofluorescence, Flow cytometry

Application notes

Immunofluorescence 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Optimal dilution for a specific application should be determined by user

Positive control

K-562 or U-937 cells. Lymph nodes and tonsils.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Immunology

Cell/tissue expression

Monocytes/macrophages, Neutrophils, NK cells

Conjugation
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Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

This antibody recognizes CD16 (FcgammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (FcgammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (FcgammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

References

Nemec M et al. Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang 52:125-8 (1987)

 

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