Monoclonal anti CD16 (HuNK2)

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Product Description

Recognizes a protein of 50-65kDa, identified as CD16 (Workshop IV; Code N39 ) (also known low affinity Fc receptor III for IgG (FcRIII) or Leu 11). CD16 exists as a polypepetide-anchored from (FCRIIIA or CD16A) on human natural killer (NK) cells and monocytes/ macrophages and as a glycosylphosphatidylinositol (GPI)-anchored form (FcRIIIB or CD16B) on neutrophils. CD16B is polymorphic and the two alleles are termed NA1 and NA2.3 CD16 plays a role in signal transduction, NK cell activation and antibody-dependent cellular cytotoxicity. This MAb has been showed to inhibit the binding of immune complex to NK cells, inhibit cytotoxicity of NK cells, and induce calcium fluxes in NK cells and neutrophils.

Knapp W. et al. (eds) Leukocyte Typing IV, Oxford University Press, Oxford, 1989. | Lanier LL et al. Functional properties of a unique subset of cytotoxic CD3+ T lymphocytes that express Fc receptors for IgG (CD16/Leu-11 antigen). J Exp Med 1985, 162(6):2089-2106. | Nagarajan S et al. Ligand binding and phagocytosis by CD16 (Fc gamma receptor III) isoforms. Phagocytic signaling by associated zeta and gamma subunits in Chinese hamster ovary cells. J Biol Chem 1995, 270(43):25762-25770 | McKenzie SE and Schreiber AD. Biological advances and clinical applications of Fc receptors for IgG. Curr Opin Hematol 1994, 1(1):45-52. | Cerboni C et al. CD16-mediated activation of phosphatidylinositol-3 kinase (PI-3K) in human NK cells involves tyrosine phosphorylation of Cbl and its association with Grb2, Shc, pp36 and p85 PI-3K subunit. Eur J Immunol 1998, 28(3):1005-1015. | Braakman E et al. CD16 on human ????T lymphocytes: expression, function, and specificity for mouse IgG isotypes. Cell Immunol 1992, 143:97-107

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

CD16 (HuNK2)

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone

HuNK2

Molecular weight

50-80 kDa

Synonyms
Entrez gene ID

2214

SwissProt

P08637

Unigene

372679

Immunogen

Human peripheral blood lymphocytes from a NK-leukemia patient.

Species reactivity

Human

Applications

Functional studies, Flow cytometry, Immunofluorescence

Cellular localization

Cell surface

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Functional Studies order Ab without BSA & azide), Immunofluorescence 0.5-1 ug/mL, Optimal dilution for a specific application should be determined

Positive control

K-562 or U-937 cells. Lymph nodes and tonsils.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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